Sarcosine Oxidase Enzyme for Creatinine Assay >=12 U/mg 40 kDa Stabilized by Potassium Ions CAS 9029-22-5

Name: Sarcosine Oxidase Enzyme for Creatinine Assay >=12 U/mg 40 kDa Stabilized by Potassium Ions CAS 9029-22-5
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Cat.No: IEC-HMM-0284 Datasheet

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Product Name	Sarcosine Oxidase Enzyme for Creatinine Assay >=12 U/mg 40 kDa Stabilized by Potassium Ions CAS 9029-22-5
Catalog No.	IEC-HMM-0284
Description	Sarcosine oxidase from Alta DiagnoTech catalyzes the oxidative demethylation of sarcosine to glycine, formaldehyde and hydrogen peroxide. It is a critical component of enzymatic creatinine test systems.
Intended Use	Raw enzyme for creatinine assay kits and sarcosine detection reagents.
Principle / Technology	Oxidizes sarcosine to glycine, formaldehyde and H2O2; the H2O2 is detected via chromogenic peroxidase coupling.
Detection Method	Colorimetric peroxidase-coupled assay.
Sample Type	Serum, plasma, urine.
Performance Range / Specifications	Specific activity >=12 U/mg. Molecular weight 40 kDa. Isoelectric point 5.5.
Components / Formulation	Sarcosine oxidase lyophilized powder.
Storage Conditions	-20°C.
Shelf Life	Stable for at least 12 months at -20°C.
Package Specifications	KU packaging units.
Product Form	Yellow amorphous powder.
Purity	99%
Activity / Unit Definition	>=12 U/mg
Molecular Weight	40 kDa
Source / Origin	Microbial fermentation.
pH Range / Optimal pH	Optimal pH 7.0-8.0; stable between pH 6.5-10.5 at 20°C for 24 hours.
Shipping Conditions	Cold chain shipment.
Expiration Date / Stability	-20°C stable for one year.
Appearance	Yellow amorphous powder.
CAS Number	9029-22-5
Isoelectric Point	5.5
Inhibitors	Not inhibited by sodium azide.
Optimal Temperature	50-55°C.
Thermal Stability	No loss of activity below 60°C for 10 minutes.
Handling Precaution	Reconstitute immediately before use; potassium ions improve stability.
Michaelis Constant	2.09×10^-3 mol/L (Sarcosine)
Stabilizer	Potassium ions help stabilize enzyme activity.

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For research use only, not for clinical use.