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| Product Name | Ribonuclease A (RNase A), from Bovine Pancreas |
| Catalog No. | IVDRM-0185 |
| Description | Ribonuclease A (RNase A) is a single-chain polypeptide containing four disulfide bonds, with a molecular weight of approximately 13.7 kDa (based on the amino acid sequence). As an endoribonuclease, it specifically degrades RNA at cytosine (C) and uracil (U) residues. RNase A shows the highest activity on single-stranded RNA and is compatible with a wide range of reaction systems at a recommended working concentration of 1-100 μg/mL. At low salt concentrations (0-100 mM NaCl), it cleaves single-stranded RNA, double-stranded RNA, and the RNA strand of RNA-DNA hybrids; at high salt concentrations (≥0.3 M), it cleaves single-stranded RNA only. The most common application of RNase A is the removal of RNA during plasmid DNA or genomic DNA preparation; contaminating DNase activity is a key concern in such preparations and is traditionally inactivated by boiling in a water bath. This product is also suitable for RNase protection assays, RNA sequence analysis, and other molecular biology experiments. |
| Intended Use | For removal of RNA during plasmid DNA or genomic DNA preparation; also suitable for RNase protection assays, RNA sequence analysis, and other molecular biology experiments. |
| Principle / Technology | RNase A is an endoribonuclease that specifically degrades RNA at cytosine (C) and uracil (U) residues. It cleaves the phosphodiester bond formed between the 5'-ribose of one nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide, hydrolyzing the 2',3'-cyclic phosphate intermediate to the corresponding 3'-nucleoside phosphate (for example, pG-pG-pC-pA-pG is cleaved by RNase A to yield pG-pG-pCp and A-pG). |
| Sample Type | Single-stranded RNA; under low-salt conditions also double-stranded RNA and the RNA strand of RNA-DNA hybrids. |
| Performance Range / Specifications | Activity ≥80 Kunitz U/mg. Highest activity on single-stranded RNA; recommended working concentration 1-100 μg/mL, compatible with a wide range of reaction systems. |
| Specificity | Specifically degrades RNA at cytosine (C) and uracil (U) residues of single-stranded RNA; at high salt concentrations (≥0.3 M NaCl), cleaves single-stranded RNA only. |
| Reaction Conditions / Protocol | Recommended working concentration: 1-100 μg/mL; compatible with a wide range of reaction systems. At low salt concentrations (0-100 mM NaCl), cleaves single-stranded RNA, double-stranded RNA, and the RNA strand of RNA-DNA hybrids; at high salt concentrations (≥0.3 M), cleaves single-stranded RNA only. |
| Storage Conditions | Store dry at -25°C to -15°C. |
| Shelf Life | 3 years. |
| Package Specifications | 100 mg; 1 g; 5 g; 10 g |
| Product Form | Powder. |
| Activity / Unit Definition | Activity ≥80 Kunitz U/mg. One unit is defined as the amount of enzyme that hydrolyzes RNA at a rate constant k equal to unity (Kunitz units) at 25°C and pH 5.0. |
| Molecular Weight | Approximately 13.7 kDa (amino acid sequence) |
| Source / Origin | Bovine pancreas. |
| Shipping Conditions | Shipped with ice packs. |
For research use only, not for clinical use.
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