- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Reverse Transcriptase III (600 U/μL, Glycerol-Free) |
| Catalog No. | IVDRM-0246 |
| Description | This enzyme is a new-generation reverse transcriptase obtained by genetic engineering of an RNase H-deficient M-MLV reverse transcriptase. Compared with the parental enzyme, its thermostability is greatly improved, tolerating reaction temperatures up to 65 °C, which makes it suitable for reverse transcription of RNA templates with complex secondary structures. The enzyme also shows enhanced template affinity, making it suitable for small amounts of template and low-copy genes. Its ability to synthesize full-length cDNA is improved, enabling amplification of cDNA up to 19.8 kb. This glycerol-free version (600 U/μL) can be used to prepare lyophilized formulations, such as lyophilizable RT-LAMP reagents. |
| Intended Use | Raw material for IVD molecular diagnostic reagents; suitable for the preparation of lyophilized formulations, including lyophilizable RT-LAMP reagents. |
| Principle / Technology | Genetically engineered M-MLV (H-) reverse transcriptase with high thermostability and enhanced template affinity, recombinantly expressed. |
| Detection Method | RT-PCR / RT-qPCR / RT-LAMP |
| Sample Type | RNA templates, including RNA with complex secondary structures and low-abundance/low-copy templates |
| Performance Range / Specifications | Supports reverse transcription at up to 65 °C Amplifies cDNA up to 19.8 kb Glycerol-free formulation, suitable for lyophilized formulations and lyophilizable RT-LAMP reagents |
| Specificity | Enhanced affinity for low-abundance templates and low-copy genes |
| Reaction Conditions / Protocol | Reverse transcription can be performed at elevated temperatures up to 65 °C for RNA templates with complex secondary structures. |
| Components / Formulation | Glycerol-free liquid enzyme formulation, 600 U/μL |
| Storage Conditions | Store at 4 °C. |
| Shelf Life | 6 months |
| Package Specifications | 6 KU 12 KU 300 KU |
| Product Form | Glycerol-free liquid |
| Concentration | 600 U/μL |
| Source / Origin | Genetically engineered M-MLV (H-) reverse transcriptase, recombinantly expressed |
| Shipping Conditions | Shipped with ice packs. |
| Expiration Date / Stability | Valid for 6 months when stored at 4 °C |
| Compatibility | Suitable for lyophilized reagent manufacturing, including lyophilizable RT-LAMP reagents |
| Application Notes / Precautions | For RNA templates with complex secondary structures, perform reverse transcription at elevated temperature (up to 65 °C). The glycerol-free formulation supports lyophilization. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
