- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Restriction Enzyme XmnI (Recognition Site: 5'-GAANN↓NNTTC-3') |
| Catalog No. | IVDRM-0161 |
| Description | XmnI is a Type IIP restriction endonuclease originally derived from Xanthomonas manihotis, produced by recombinant expression in E. coli and purified for reliable performance. It recognizes and cleaves the GAANN/NNTTC sequence, generating blunt ends, and delivers its best performance and star-activity control in Cut Buffer C. XmnI is also compatible with the universal reaction buffer, making it easy to pair with other endonucleases for rapid double digestion; however, prolonged digestion should be avoided to prevent star activity. |
| Intended Use | Site-specific cleavage of DNA at GAANN/NNTTC recognition sites to generate blunt ends for molecular cloning and restriction analysis, including double digestion with other restriction enzymes. |
| Principle / Technology | Type IIP restriction endonuclease that recognizes the interrupted palindromic sequence 5'-GAANN↓NNTTC-3' and cleaves both strands to produce blunt ends. |
| Sample Type | Double-stranded DNA substrates such as λDNA. |
| Performance Range / Specifications | Concentration: 10 U/μL Generates blunt ends Optimal performance and star-activity control in Cut Buffer C Compatible with the universal reaction buffer for double digestion |
| Specificity | Specifically recognizes and cleaves 5'-GAANN↓NNTTC-3' / 3'-CTTNN↑NNAAG-5', producing blunt ends. |
| Reaction Conditions / Protocol | 1×Cut Buffer C; incubate at 37°C. Avoid prolonged digestion to prevent star activity. Heat inactivation: 80°C for 20 min. |
| Components / Formulation | XmnI restriction enzyme (10 U/μL); 1×Cut Buffer C reaction buffer. |
| Storage Conditions | Store at -25 to -15°C. |
| Shelf Life | 2 years when stored at -25 to -15°C. |
| Package Specifications | 500 U |
| Concentration | 10 U/μL |
| Activity / Unit Definition | One unit (U) is defined as the amount of enzyme required to completely digest 1 μg of λDNA in a 50 μL reaction at 37°C within 1 h. |
| Source / Origin | Derived from Xanthomonas manihotis; recombinantly expressed in E. coli and purified. |
| Expiration Date / Stability | Stable for 2 years at -25 to -15°C. |
| Recommended Buffer System | 1×Cut Buffer C (optimal performance and star-activity control); compatible with the universal reaction buffer for double digestion with other endonucleases. |
| Application Notes / Precautions | Use Cut Buffer C for optimal performance and star-activity control. Avoid prolonged digestion to prevent star activity. Compatible with the universal reaction buffer for rapid double digestion with other endonucleases. |
| Recognition Site | 5'-GAANN↓NNTTC-3' 3'-CTTNN↑NNAAG-5' |
| Heat Inactivation | 80°C for 20 min. |
| Methylation Sensitivity | CpG: cleavage affected. EcoBI: cleavage blocked. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
