Restriction Enzyme SwaI (Recognition Site: 5'-ATTT↓AAAT-3')

Name: Restriction Enzyme SwaI (Recognition Site: 5'-ATTT↓AAAT-3')
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Cat.No: IVDRM-0225 Datasheet

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Product Name	Restriction Enzyme SwaI (Recognition Site: 5'-ATTT↓AAAT-3')
Catalog No.	IVDRM-0225
Description	SwaI is a Type IIP restriction endonuclease originally derived from Staphylococcus warneri and produced by recombinant expression in E. coli. It recognizes and cleaves the 8-bp palindromic sequence 5'-ATTTAAAT-3', generating blunt ends. It is widely used in molecular cloning, genotyping, and related research applications.
Intended Use	For molecular cloning, genotyping, and other molecular biology research applications. For research use only.
Principle / Technology	Type IIP restriction endonuclease; sequence-specific double-stranded DNA cleavage at a palindromic recognition site, producing blunt ends.
Detection Method	Cleavage products are typically analyzed by agarose gel electrophoresis.
Sample Type	Double-stranded DNA (e.g., plasmid DNA, PCR products).
Specificity	Isoschizomer: SmiI. Cleaves the 8-bp palindrome 5'-ATTTAAAT-3' to generate blunt ends.
Reaction Conditions / Protocol	1x Cut Buffer C; incubate at 37°C.
Storage Conditions	Store at -25°C to -15°C.
Shelf Life	2 years when stored as recommended.
Package Specifications	1000 U
Concentration	10 U/uL
Activity / Unit Definition	One unit (U) is defined as the amount of enzyme required to completely digest 1 ug of V plasmid DNA in 1 hour at 37°C in a 50 uL reaction.
Source / Origin	Derived from Staphylococcus warneri; recombinantly expressed in E. coli.
Expiration Date / Stability	Stable for 2 years at -25°C to -15°C.
Recommended Buffer System	1x Cut Buffer C
Recognition Site	5'-ATTT↓AAAT-3' 3'-TAAA↑TTTA-5'
Heat Inactivation	Incubate at 80°C for 20 min.
Methylation Sensitivity	Not affected by methylation.

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For research use only, not for clinical use.