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| Product Name | Restriction Enzyme SspDI (KasI) (Recognition Site: 5'-G↓GCGCC-3') |
| Catalog No. | IVDRM-0160 |
| Description | SspDI (KasI) is a conventional restriction endonuclease that recognizes the G/GCGCC sequence. Unlike rapid restriction enzymes, SspDI (KasI) requires a longer digestion time to achieve complete cleavage of DNA substrates, but it uses the same universal reaction buffer as the rapid-enzyme series, making it easy to combine with other restriction enzymes in double digestions. |
| Intended Use | Site-specific cleavage of DNA at G/GCGCC recognition sites for molecular cloning and restriction analysis, including double digestion with other restriction enzymes. |
| Principle / Technology | Type II restriction endonuclease that recognizes the palindromic sequence 5'-G↓GCGCC-3' and cleaves double-stranded DNA at defined positions within the site. |
| Sample Type | Double-stranded DNA substrates such as pBR322. |
| Performance Range / Specifications | Concentration: 10 U/μL Requires extended digestion time for complete cleavage of DNA substrates Isoschizomers: KasI, DinI, EgeI, EheI, Mly113I, NarI, PluTI, SfoI |
| Specificity | Specifically recognizes and cleaves 5'-G↓GCGCC-3' / 3'-CCGCG↑G-5'. |
| Reaction Conditions / Protocol | 1× universal reaction buffer; incubate at 37°C. Allow a longer digestion time for complete cleavage. Heat inactivation: 80°C for 20 min. |
| Components / Formulation | SspDI (KasI) restriction enzyme (10 U/μL); universal reaction buffer. |
| Storage Conditions | Store at -25 to -15°C. |
| Shelf Life | 2 years when stored at -25 to -15°C. |
| Package Specifications | 250 U |
| Concentration | 10 U/μL |
| Activity / Unit Definition | One unit (U) is defined as the amount of enzyme required to completely digest 1 μg of pBR322 DNA in a 50 μL reaction at 37°C within 1 h. |
| Expiration Date / Stability | Stable for 2 years at -25 to -15°C. |
| Compatibility | Isoschizomers: KasI, DinI, EgeI, EheI, Mly113I, NarI, PluTI, SfoI. |
| Recommended Buffer System | 1× universal reaction buffer (shared with the rapid restriction enzyme series). |
| Application Notes / Precautions | SspDI (KasI) is a conventional (non-rapid) enzyme; extend the digestion time to ensure complete cleavage. Uses the universal reaction buffer, enabling double digestion with other restriction enzymes. |
| Recognition Site | 5'-G↓GCGCC-3' 3'-CCGCG↑G-5' |
| Heat Inactivation | 80°C for 20 min. |
| Methylation Sensitivity | CpG: cleavage blocked. Dam, Dcm, EcoKI, EcoBI: no effect. |
For research use only, not for clinical use.
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