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| Product Name | Restriction Enzyme PciI (Recognition Site: 5'-A↓CATGT-3') |
| Catalog No. | IVDRM-0157 |
| Description | PciI is a Type IIP restriction endonuclease originally derived from Planococcus citreus and obtained through recombinant expression in E. coli. Used with its dedicated reaction buffer, PciI delivers rapid digestion; it is also compatible with the universal digestion buffer for double digestions, although prolonged incubation may lead to star activity. |
| Intended Use | Rapid restriction digestion of DNA; suitable for double-digest workflows. |
| Principle / Technology | As a Type IIP restriction enzyme, PciI recognizes the palindromic sequence ACATGT and cleaves between A and C on the top strand (and at the symmetric position on the bottom strand) to generate defined ends. |
| Sample Type | DNA substrates (e.g., p615 DNA). |
| Performance Range / Specifications | Concentration: 10 U/μL. Optimal reaction temperature: 37°C. One unit (U) completely digests 1 µg of p615 DNA in 1 h at 37°C in a 50 μL reaction. Rapid digestion with the dedicated buffer; star activity may occur during prolonged incubation. |
| Specificity | Specifically recognizes the palindromic sequence 5'-ACATGT-3' and cleaves at 5'-A↓CATGT-3'. |
| Reaction Conditions / Protocol | 1× Cut Buffer C; incubate at 37°C. Heat inactivation: 80°C for 20 min. |
| Components / Formulation | PciI restriction enzyme (10 U/μL) with a dedicated 1× Cut Buffer C for rapid digestion. |
| Storage Conditions | Store at -25 to -15°C. |
| Shelf Life | 2 years. |
| Package Specifications | 200 U |
| Concentration | 10 U/μL |
| Activity / Unit Definition | One unit (U) is defined as the amount of enzyme required to completely digest 1 µg of p615 DNA in 1 h at 37°C in a 50 μL reaction system. |
| Source / Origin | Derived from Planococcus citreus; obtained by recombinant expression in E. coli. |
| Expiration Date / Stability | Valid for 2 years when stored at -25 to -15°C. |
| Compatibility | Compatible with the universal digestion buffer for double digestions; prolonged reactions may show star activity. |
| Recommended Buffer System | 1× Cut Buffer C. |
| Recognition Site | 5'-A↓CATGT-3' 3'-TGTAC↑A-5' |
| Heat Inactivation | 80°C for 20 min. |
| Methylation Sensitivity | EcoKI, EcoBI: cleavage blocked. |
For research use only, not for clinical use.
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