Restriction Enzyme BstXI (Recognition Site: 5'-CCA(N)5↓NTGG-3')

Name: Restriction Enzyme BstXI (Recognition Site: 5'-CCA(N)5↓NTGG-3')
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Cat.No: IVDRM-0156 Datasheet

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Product Name	Restriction Enzyme BstXI (Recognition Site: 5'-CCA(N)5↓NTGG-3')
Catalog No.	IVDRM-0156
Description	BstXI is a Type IIP restriction enzyme that recognizes a palindromic sequence. The optimized reaction buffer enables BstXI to perform at its maximum activity, and the buffer contains recombinant albumin, which enhances the stability of the enzyme.
Intended Use	Molecular cloning.
Principle / Technology	As a Type IIP restriction enzyme, BstXI recognizes the palindromic sequence CCA(N)5NTGG and cleaves within the recognition sequence to generate defined ends.
Sample Type	DNA substrates (e.g., λ DNA).
Performance Range / Specifications	Concentration: 10 U/μL. Optimal reaction temperature: 37°C. One unit (U) completely digests 1 µg of λ DNA in 1 h at 37°C in a 50 μL reaction.
Specificity	Specifically recognizes the palindromic sequence 5'-CCA(N)5NTGG-3'.
Reaction Conditions / Protocol	1× BstXI Buffer; incubate at 37°C. Heat inactivation: 80°C for 20 min.
Components / Formulation	BstXI restriction enzyme (10 U/μL) with an optimized reaction buffer containing recombinant albumin for enhanced enzyme stability.
Storage Conditions	Store at -25 to -15°C.
Shelf Life	2 years.
Package Specifications	500 U
Concentration	10 U/μL
Activity / Unit Definition	One unit (U) is defined as the amount of enzyme required to completely digest 1 µg of λ DNA in 1 h at 37°C in a 50 μL reaction system.
Expiration Date / Stability	Valid for 2 years when stored at -25 to -15°C.
Recommended Buffer System	1× BstXI Buffer.
Recognition Site	5'-CCA(N)5↓NTGG-3' 3'-GGTN↑(N)5ACC-5'
Heat Inactivation	80°C for 20 min.
Methylation Sensitivity	Dcm: cleavage affected. Dam, CpG, EcoKI, EcoBI: no effect.

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For research use only, not for clinical use.