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| Product Name | Restriction Enzyme BsrDI (Recognition Site: 5'-GCAATGNN↓-3') |
| Catalog No. | IVDRM-0155 |
| Description | BsrDI, derived from Bacillus stearothermophilus D70, is a Type IIS restriction enzyme that recognizes the sequence GCAATG/CATTGC. It cleaves the top strand two nucleotides downstream of the recognition site and the bottom strand immediately after the recognition site, generating specific cohesive ends, and can be used for Golden Gate assembly. |
| Intended Use | Molecular cloning and Golden Gate assembly. |
| Principle / Technology | As a Type IIS restriction enzyme, BsrDI recognizes the specific sequence GCAATG/CATTGC and cleaves outside the recognition site—two nucleotides downstream on the top strand and directly after the recognition site on the bottom strand—to produce specific cohesive (sticky) ends. |
| Sample Type | DNA substrates (e.g., λ DNA). |
| Performance Range / Specifications | Concentration: 10 U/μL. Optimal reaction temperature: 37°C. One unit (U) completely digests 1 µg of λ DNA in 1 h at 37°C in a 50 μL reaction. |
| Specificity | Specifically recognizes 5'-GCAATG-3' and cleaves outside the recognition sequence to generate defined sticky ends. |
| Reaction Conditions / Protocol | 1× BsrDI Buffer; incubate at 37°C. Heat inactivation: 80°C for 20 min. |
| Components / Formulation | BsrDI restriction enzyme (10 U/μL) with a dedicated 1× BsrDI reaction buffer. |
| Storage Conditions | Store at -25 to -15°C. |
| Shelf Life | 2 years. |
| Package Specifications | 250 U |
| Concentration | 10 U/μL |
| Activity / Unit Definition | One unit (U) is defined as the amount of enzyme required to completely digest 1 µg of λ DNA in 1 h at 37°C in a 50 μL reaction system. |
| Source / Origin | Derived from Bacillus stearothermophilus D70. |
| Expiration Date / Stability | Valid for 2 years when stored at -25 to -15°C. |
| Compatibility | Isoschizomers: Bse3DI, BseMI. |
| Recommended Buffer System | 1× BsrDI Buffer. |
| Recognition Site | 5'-GCAATGNN↓-3' 3'-CGTTAC↑NN-5' |
| Heat Inactivation | 80°C for 20 min. |
| Methylation Sensitivity | EcoBI: cleavage blocked. |
For research use only, not for clinical use.
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