Restriction Enzyme BspQI (Recognition Site: 5'-GCTCTTC(N)↓-3')

Name: Restriction Enzyme BspQI (Recognition Site: 5'-GCTCTTC(N)↓-3')
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Cat.No: IVDRM-0154 Datasheet

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Product Name	Restriction Enzyme BspQI (Recognition Site: 5'-GCTCTTC(N)↓-3')
Catalog No.	IVDRM-0154
Description	BspQI is a Type II restriction endonuclease supplied as a recombinant protein encoded by the BspQI gene from Bacillus sphaericus and expressed in E. coli. It recognizes the sequence 5'-GCTCTTCN1/N4-3' and is mainly used to linearize plasmids for the preparation of linear DNA fragments ending in poly(A/T/G/C) tails, yielding specific cohesive ends.
Intended Use	Digestion of plasmids to prepare linearized DNA fragments with poly(A/T/G/C) ends and specific cohesive ends.
Principle / Technology	As a Type II restriction enzyme, BspQI recognizes the sequence GCTCTTC and cleaves at fixed positions downstream of the recognition site (1 nt on the top strand and 4 nt on the bottom strand), generating defined cohesive ends.
Sample Type	DNA substrates (e.g., plasmid DNA).
Performance Range / Specifications	Concentration: 10 U/μL. Optimal reaction temperature: 50°C.
Specificity	Specifically recognizes 5'-GCTCTTC-3' and cleaves downstream of the recognition sequence to produce defined cohesive ends.
Reaction Conditions / Protocol	1× Digestion Buffer 3 (supplied separately); incubate at 50°C. Heat inactivation: 80°C for 20 min.
Components / Formulation	BspQI restriction enzyme (10 U/μL); 1× Digestion Buffer 3 is required and supplied separately.
Storage Conditions	Store at -20°C.
Shelf Life	1 year.
Package Specifications	500 U; 2500 U; 10000 U
Concentration	10 U/μL
Source / Origin	Recombinant protein encoded by the BspQI gene from Bacillus sphaericus, expressed in E. coli.
Shipping Conditions	Shipped on dry ice.
Expiration Date / Stability	Valid for 1 year when stored at -20°C.
Recommended Buffer System	1× Digestion Buffer 3 (supplied separately).
Recognition Site	5'-GCTCTTC(N)↓-3' 3'-CGAGAAG(N)4↑-5'
Heat Inactivation	80°C for 20 min.
Methylation Sensitivity	No effect from methylation.

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For research use only, not for clinical use.