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| Product Name | Restriction Enzyme BsiWI (Recognition Site: 5'-C↓GTACG-3') |
| Catalog No. | IVDRM-0152 |
| Description | BsiWI is a Type IIP restriction endonuclease that recognizes a palindromic sequence. The optimized reaction buffer allows BsiWI to perform at its full potential, and the inclusion of recombinant albumin in the buffer enhances the stability of a wide range of enzymes. |
| Intended Use | Molecular cloning and restriction digestion applications requiring cleavage at the palindromic sequence CGTACG. |
| Principle / Technology | BsiWI is a Type IIP restriction endonuclease that recognizes the palindromic sequence 5'-C↓GTACG-3' and cleaves within the site, generating sticky ends. The optimized reaction buffer, supplemented with recombinant albumin, maximizes enzyme performance and stability. |
| Sample Type | DNA substrates such as plasmid p615 bearing BsiWI recognition sites. |
| Performance Range / Specifications | Activity: 10 U/μL. Unit definition: One unit (U) is the amount of enzyme required to completely digest 1 µg of p615 DNA in 1 hour at 55°C in a 50 µL reaction. Methylation effects: CpG methylation blocks cleavage; Dam, Dcm, EcoKI, and EcoBI methylation have no effect. |
| Specificity | Specifically recognizes the palindromic sequence 5'-C↓GTACG-3' (complementary strand 3'-GCATG↑C-5'). |
| Reaction Conditions / Protocol | Optimal reaction: incubate at 55°C in 1× BsiWI Buffer. Heat inactivation: 80°C for 20 min. |
| Components / Formulation | BsiWI restriction enzyme (10 U/μL) and 1× BsiWI Buffer containing recombinant albumin for enhanced enzyme stability. |
| Storage Conditions | Store at -25°C to -15°C. |
| Shelf Life | Valid for 2 years when stored at -25°C to -15°C. |
| Package Specifications | 300 U |
| Concentration | 10 U/μL |
| Activity / Unit Definition | One unit (U) is defined as the amount of enzyme required to completely digest 1 µg of p615 DNA in 1 hour at 55°C in a 50 µL reaction system. |
| Expiration Date / Stability | Stable for 2 years from the date of manufacture when stored at -25°C to -15°C. |
| Compatibility | Isoschizomers: PspLI, Pfl23II. |
| Recommended Buffer System | 1× BsiWI Buffer (supplied, contains recombinant albumin). |
| Application Notes / Precautions | Perform digestions at the optimal reaction temperature of 55°C. CpG methylation of the recognition site blocks cleavage; Dam, Dcm, EcoKI, and EcoBI methylation do not affect activity. Heat-inactivate at 80°C for 20 min before downstream applications. |
| Recognition Site | 5'-C↓GTACG-3' 3'-GCATG↑C-5' |
| Heat Inactivation | 80°C for 20 min. |
| Methylation Sensitivity | CpG methylation: cleavage is blocked. Dam, Dcm, EcoKI, EcoBI methylation: no effect. |
For research use only, not for clinical use.
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