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| Product Name | Recombinant Tritirachium album Proteinase K (PROK), Active |
| Catalog No. | IEC-HMM-0133 |
| Description | An active recombinant serine protease from Tritirachium album with broad substrate specificity. The enzyme is supplied as a lyophilized powder and is suitable for protein digestion and nucleic-acid sample preparation. |
| Intended Use | For proteolysis, protein digestion, nucleic acid purification, and laboratory sample preparation. |
| Principle / Technology | Recombinant serine protease is produced and purified, and catalytic activity is quantified by tyrosine release under defined pH and temperature conditions. |
| Detection Method | SDS-PAGE; tyrosine-release activity assay at 275 nm; agarose gel electrophoresis |
| Sample Type | Purified recombinant protein |
| Performance Range / Specifications | Purity: >=95% as determined by SDS-PAGE. Molecular weight: 28.9 kDa. Package sizes: 20 mg; 100 mg; 1 g; 1 kg. |
| Sensitivity / LOD | >30 U/mg. One unit catalyzes the formation of 1 umol of tyrosine per minute at pH 7.5 and 37 C. |
| Specificity | Preferentially cleaves peptide and ester bonds adjacent to the C-termini of hydrophobic, aliphatic, or aromatic amino acids. |
| Reaction Conditions / Protocol | >30 U/mg. One unit catalyzes the formation of 1 umol of tyrosine per minute at pH 7.5 and 37 C. |
| Components / Formulation | Liquid preparations use a Tris- or PBS-based buffer with 5-50% glycerol; lyophilized preparations use a Tris- or PBS-based buffer with 6% trehalose. |
| Storage Conditions | Store at -20 C. Avoid repeated freeze-thaw cycles after reconstitution. |
| Shelf Life | Lyophilized material is stable for 24 months at -20 C or -80 C, 18 months at 4 C, and 12 months at room temperature. Powder stored at 4 C retained more than 95% activity for over one year. |
| Package Specifications | 20 mg; 100 mg; 1 g; 1 kg |
| Product Form | Lyophilized powder |
| Quality Control | Purity evaluated by SDS-PAGE. Agarose gel electrophoresis confirmed no detectable nucleic acid, DNase, or RNase residue. Enzymatic activity was measured by tyrosine release. |
| Key Features | Active recombinant protein; ; ; Recombinant Tritirachium album protein. |
| Purity | >=95% as determined by SDS-PAGE. |
| Activity / Unit Definition | >30 U/mg. One unit catalyzes the formation of 1 umol of tyrosine per minute at pH 7.5 and 37 C. |
| Molecular Weight | 28.9 kDa |
| Source / Origin | Recombinant Tritirachium album protein |
| pH Range / Optimal pH | Optimal activity definition at pH 7.5. |
| Expiration Date / Stability | Lyophilized material is stable for 24 months at -20 C or -80 C, 18 months at 4 C, and 12 months at room temperature. Powder stored at 4 C retained more than 95% activity for over one year. |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | Suitable for broad proteolysis, protein digestion, nucleic acid purification, and sample preparation workflows. |
| Recommended Buffer System | Liquid preparations use a Tris- or PBS-based buffer with 5-50% glycerol; lyophilized preparations use a Tris- or PBS-based buffer with 6% trehalose. |
| Application Notes / Precautions | Briefly centrifuge before opening and reconstitute with sterile deionized water. For storage, add 5-50% glycerol as appropriate and divide into aliquots. Avoid repeated freezing and thawing. A working aliquot may be kept at 4 C for up to one week. |
| Recognition Sequence / Cleavage Site | Preferentially cleaves peptide and ester bonds adjacent to the C-termini of hydrophobic, aliphatic, or aromatic amino acids. |
| Alternative Names / Synonyms | Endopeptidase K; Tritirachium alkaline proteinase |
| Lead Time | 3-7 business days |
| Availability Status | In Stock |
| Host Species | Tritirachium album |
| UniProt Accession | P06873 |
For research use only, not for clinical use.
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