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| Product Name | Recombinant Kex2 Protease, Lyophilized |
| Catalog No. | IEC-HMM-0120 |
| Description | A high-purity, calcium-dependent recombinant endoprotease that selectively cleaves peptide bonds after paired basic residues and is supplied as a stable lyophilized powder. |
| Intended Use | Fusion-tag removal and processing of insulin analogs, GLP-1 products and recombinant peptides, including workflows that combine Kex2 protease with carboxypeptidase B. |
| Principle / Technology | The enzyme recognizes dibasic sequences such as Arg-Arg and Lys-Arg and cleaves the peptide bond on the carboxyl side. It does not cleave after a single basic residue and is not inhibited by aprotinin, PMSF or TPCK. |
| Performance Range / Specifications | Recommended reconstituted enzyme concentration: approximately 1-10 mg/mL. |
| Specificity | Selective cleavage after paired basic amino acid residues; no cleavage after an isolated basic residue. |
| Reaction Conditions / Protocol | Use 50 mM Tris-HCl with 2 mM Ca2+ at pH 7.0-9.0 as the reaction buffer. For storage after reconstitution, dissolve in 20 mM sodium acetate-acetic acid with 2 mM Ca2+ at pH 5.2, aliquot and freeze below -20°C. |
| Components / Formulation | Lyophilized recombinant Kex2 protease. |
| Storage Conditions | Store and transport sealed and protected from light at 2-8°C. After reconstitution, aliquot and store below -20°C. |
| Shelf Life | Lyophilized powder is stable for 24 months at 2-8°C. Reconstituted enzyme is stable for 12 months below -20°C when properly aliquoted. |
| Package Specifications | 100 µg; 1 mg; 10 mg; 100 mg; 1 g; 10 g. Custom package sizes are available. |
| Product Form | Lyophilized recombinant protease. |
| Quality Control | High-purity, high-specific-activity recombinant preparation; GMP-grade material is available. |
| Key Features | High purity and specific activity; animal-origin-free production; stable lyophilized format; scalable supply; customizable packaging. |
| Purity | High purity. |
| Source / Origin | Recombinantly expressed in Pichia pastoris. |
| pH Range / Optimal pH | Reaction pH 7.0-9.0; preferred storage pH 5.0-6.0. |
| Shipping Conditions | Ship at 2-8°C in sealed, light-protected packaging. |
| Regulatory / Compliance | GMP-grade product can be supplied. |
| Compatibility | Compatible with peptide and fusion-protein processing workflows, including sequential processing with carboxypeptidase B. |
| Recommended Buffer System | Reaction buffer: 50 mM Tris-HCl, 2 mM Ca2+, pH 7.0-9.0. Reconstitution/storage buffer: 20 mM sodium acetate-acetic acid, 2 mM Ca2+, pH 5.2. |
| Application Notes / Precautions | Avoid repeated freeze-thaw cycles after reconstitution. Maintain calcium in the reaction buffer for enzyme activity. |
| Batch-to-Batch Consistency | Produced for stable quality and scalable batch supply. |
| Recognition Sequence / Cleavage Site | Cleaves on the carboxyl side of paired basic residues, including Arg-Arg and Lys-Arg. |
For research use only, not for clinical use.
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