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| Product Name | Recombinant Human GOT1 Aspartate Aminotransferase, 58 kDa BSA Powder |
| Catalog No. | IEC-HMM-0153 |
| Description | Alta DiagnoTech aspartate aminotransferase is recombinant human GOT1 expressed in Escherichia coli and supplied as a pale yellow-green amorphous powder containing BSA. |
| Intended Use | Enzyme raw material for AST assay development, calibration and quality-control applications. |
| Principle / Technology | AST converts L-aspartate and alpha-ketoglutarate to oxaloacetate and L-glutamate; a coupled malate-dehydrogenase reaction monitors NADH consumption. |
| Detection Method | Coupled AST/MDH enzyme activity assay |
| Performance Range / Specifications | Concentration is provided on the product test report. |
| Storage Conditions | Stable for at least 3 months at 2-8 degrees C; store below -20 degrees C for long-term preservation |
| Product Form | Pale yellow-green amorphous powder containing BSA |
| Activity / Unit Definition | At 37 degrees C, one unit is the amount of AST that generates 1 umol L-glutamate or consumes 1 umol NADH per minute in one liter of reaction mixture. |
| Molecular Weight | Approximately 58 kDa by SDS-PAGE |
| Source / Origin | Recombinant human GOT1 expressed in Escherichia coli |
| pH Range / Optimal pH | Stable at pH 5.0-9.5 for 24 hours at 20 degrees C |
| Appearance | Pale yellow-green amorphous powder containing BSA |
| Alias | Aspartate aminotransferase |
| Safety | Contains sodium azide preservative; use appropriate precautions. No other known biological hazards are stated. |
| Product Type | Aspartate aminotransferase enzyme raw material |
| Function / Efficacy | Catalyze transfer of an amino group from L-aspartate to alpha-ketoglutarate. |
| Abbreviation | AST |
| Customization | Specifications can be customized |
| EC Number | EC 2.6.1.1 |
| Isoelectric Point | 7.9 |
| Thermal Stability | No activity loss after 30 minutes at or below 60 degrees C |
| Activator | Pyridoxal 5'-phosphate cofactor |
| Expression Host | Escherichia coli |
| Interference | Activity is not affected by 0.1% NaN3 or phosphate |
For research use only, not for clinical use.
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