Recombinant Human GOT1 Aspartate Aminotransferase, 58 kDa BSA Powder
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Recombinant Human GOT1 Aspartate Aminotransferase, 58 kDa BSA Powder

Cat.No: IEC-HMM-0153 Datasheet

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Product Name Recombinant Human GOT1 Aspartate Aminotransferase, 58 kDa BSA Powder
Catalog No. IEC-HMM-0153
Description Alta DiagnoTech aspartate aminotransferase is recombinant human GOT1 expressed in Escherichia coli and supplied as a pale yellow-green amorphous powder containing BSA.
Intended Use Enzyme raw material for AST assay development, calibration and quality-control applications.
Principle / Technology AST converts L-aspartate and alpha-ketoglutarate to oxaloacetate and L-glutamate; a coupled malate-dehydrogenase reaction monitors NADH consumption.
Detection Method Coupled AST/MDH enzyme activity assay
Performance Range / Specifications Concentration is provided on the product test report.
Storage Conditions Stable for at least 3 months at 2-8 degrees C; store below -20 degrees C for long-term preservation
Product Form Pale yellow-green amorphous powder containing BSA
Activity / Unit Definition At 37 degrees C, one unit is the amount of AST that generates 1 umol L-glutamate or consumes 1 umol NADH per minute in one liter of reaction mixture.
Molecular Weight Approximately 58 kDa by SDS-PAGE
Source / Origin Recombinant human GOT1 expressed in Escherichia coli
pH Range / Optimal pH Stable at pH 5.0-9.5 for 24 hours at 20 degrees C
Appearance Pale yellow-green amorphous powder containing BSA
Alias Aspartate aminotransferase
Safety Contains sodium azide preservative; use appropriate precautions. No other known biological hazards are stated.
Product Type Aspartate aminotransferase enzyme raw material
Function / Efficacy Catalyze transfer of an amino group from L-aspartate to alpha-ketoglutarate.
Abbreviation AST
Customization Specifications can be customized
EC Number EC 2.6.1.1
Isoelectric Point 7.9
Thermal Stability No activity loss after 30 minutes at or below 60 degrees C
Activator Pyridoxal 5'-phosphate cofactor
Expression Host Escherichia coli
Interference Activity is not affected by 0.1% NaN3 or phosphate

For research use only, not for clinical use.

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