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| Product Name | Recombinant Cystathionine Beta-Synthase, 3,000 U/L Liquid or >=350 U/g Powder |
| Catalog No. | IEC-HMM-0177 |
| Description | Alta DiagnoTech cystathionine beta-synthase is available as a bright-yellow stabilized solution or lyophilized powder for cystathionine-pathway homocysteine assays. |
| Intended Use | Key enzyme raw material for homocysteine assay kits based on the cystathionine pathway, used with cystathionine beta-lyase and D-lactate dehydrogenase. |
| Principle / Technology | The reverse CBS reaction produces L-serine and L-homocysteine from L-cystathionine; L-homocysteine reacts with DTNB to form TNB for rate measurement. |
| Detection Method | Kinetic TNB absorbance measurement at 412 nm |
| Performance Range / Specifications | Liquid activity concentration 3,000 U/L; liquid protein specific activity about 1.0 U/mg; lyophilized powder >=350 U/g; SDS-PAGE purity >80%. |
| Reaction Conditions / Protocol | Measure the reverse reaction with L-cystathionine at pH 8.6 and 37 degrees C and calculate volumetric activity from the TNB absorbance-change rate at 412 nm. |
| Components / Formulation | Bright-yellow liquid in potassium phosphate buffer containing 50% glycerol, stabilizers and 0.1% sodium azide; lyophilized powder is also available. |
| Storage Conditions | Store frozen at -20 degrees C |
| Shelf Life | 18 months at -20 degrees C |
| Product Form | Bright-yellow solution or lyophilized powder |
| Purity | >80% by SDS-PAGE |
| Activity / Unit Definition | One unit forms 1 umol L-homocysteine per minute at 37 degrees C in the pH 8.6 reverse-reaction system with L-cystathionine as substrate. |
| Molecular Weight | Approximately 57 kDa by SDS-PAGE |
| Source / Origin | Microbial gene, recombinantly expressed in E. coli |
| pH Range / Optimal pH | Stable at pH 6.0-11.0 for 24 hours at 25 degrees C; optimum pH 8.5 |
| Alias | Cystathionine beta-synthase |
| Safety | Contains preservative; use suitable contact protection. |
| Product Type | Cystathionine beta-synthase enzyme raw material |
| Function / Efficacy | Generate L-homocysteine in the reverse cystathionine reaction for coupled measurement. |
| Abbreviation | CBS |
| Customization | Specifications can be customized |
| Specific Activity | About 1.0 U/mg protein in liquid; >=350 U/g for lyophilized powder |
| Isoelectric Point | 7.02 |
| Inhibitors | Sodium azide and phosphate do not inhibit activity |
| Optimal Temperature | Activity increases over 20-60 degrees C |
| Thermal Stability | Stable below 45 degrees C with stabilizer at pH 7.5 for 30 minutes |
| Activator | 5'-pyridoxal phosphate cofactor |
| Stabilizer | Glycerol, Triton X-100 and BSA |
| Detection Wavelength | 412 nm |
For research use only, not for clinical use.
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