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| Product Name | Rapid-Activation Antibody Hot-Start Taq DNA Polymerase |
| Catalog No. | IEC-HMM-0095 |
| Description | An antibody-blocked hot-start Taq DNA polymerase with rapid 30-second activation and broad compatibility across PCR and qPCR buffer conditions. |
| Intended Use | Hot-start PCR and qPCR; amplification of low-copy targets from genomic DNA or cDNA; molecular assay development. |
| Principle / Technology | A thermostable antibody tightly blocks Taq activity at 55°C. Heating at 95°C for at least 30 seconds inactivates the antibody and fully releases polymerase activity. |
| Detection Method | Endpoint PCR or compatible fluorescent qPCR. |
| Sample Type | Genomic DNA, cDNA and other complex DNA templates. |
| Performance Range / Specifications | 500 U supplied at either 2.5 U/µL or 5 U/µL; full activation after at least 30 seconds at 95°C. |
| Sensitivity / LOD | Supports sensitive amplification of low-copy targets from complex templates. |
| Specificity | Strict antibody blocking during setup and temperature ramping suppresses nonspecific amplification. |
| Reaction Conditions / Protocol | Include an initial 95°C activation step of at least 30 seconds, followed by the assay-specific PCR or qPCR cycling program. |
| Storage Conditions | Store at −20°C. |
| Package Specifications | 500 U at 2.5 U/µL; 500 U at 5 U/µL. |
| Product Form | Antibody-blocked hot-start Taq DNA polymerase. |
| Key Features | Rapid 30-second activation; high specificity; strong sensitivity; broad buffer compatibility; 3′ A-overhang generation. |
| Concentration | 2.5 U/µL or 5 U/µL. |
| Compatibility | Compatible with diverse PCR/qPCR buffers and suitable for genomic DNA and cDNA templates. PCR products carry a 3′ A overhang suitable for T-vector cloning. |
For research use only, not for clinical use.
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