Pyruvate Kinase Enzyme for Potassium Ion Determination >=25 U/mg 60 kDa Activated by Mg2+ and K+ CAS 9001-59-6

Name: Pyruvate Kinase Enzyme for Potassium Ion Determination >=25 U/mg 60 kDa Activated by Mg2+ and K+ CAS 9001-59-6
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Cat.No: IEC-HMM-0288 Datasheet

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Product Name	Pyruvate Kinase Enzyme for Potassium Ion Determination >=25 U/mg 60 kDa Activated by Mg2+ and K+ CAS 9001-59-6
Catalog No.	IEC-HMM-0288
Description	Pyruvate kinase from Alta DiagnoTech catalyzes the transfer of a phosphate group from phosphoenolpyruvate to ADP, yielding pyruvate and ATP. It is used in potassium ion determination kits as a key coupling enzyme.
Intended Use	Enzyme raw material for potassium assay kits and glycolysis research.
Principle / Technology	Transfers phosphate from PEP to ADP; the reaction rate depends on potassium concentration, enabling indirect potassium quantification.
Detection Method	Coupled spectrophotometric assay monitoring ATP or pyruvate formation.
Sample Type	Serum, plasma.
Performance Range / Specifications	Specific activity >=25.0 U/mg. Molecular weight approximately 60 kDa. Isoelectric point 5.5.
Components / Formulation	Pyruvate kinase lyophilized powder.
Storage Conditions	-20°C.
Shelf Life	Stable for at least 12 months at -20°C.
Package Specifications	KU packaging units.
Product Form	White to pale yellow amorphous lyophilized powder.
Purity	99%
Activity / Unit Definition	>=25.0 U/mg
Molecular Weight	Approximately 60 kDa
Source / Origin	Microbial fermentation.
pH Range / Optimal pH	Optimal pH 8.0; stable between pH 5.0-9.5.
Shipping Conditions	Cold chain shipment.
Expiration Date / Stability	-20°C stable for one year.
Appearance	White to pale yellow amorphous lyophilized powder.
CAS Number	9001-59-6
Isoelectric Point	5.5
Inhibitors	Not inhibited by sodium azide.
Optimal Temperature	50°C.
Thermal Stability	Stable below 50°C.
Handling Precaution	Reconstitute in buffer containing Mg2+ and K+ activators.
Michaelis Constant	3.6×10^-4 M (ADP·K); 9.1×10^-4 M (PEP·K)
Activator	Mg2+ and K+ are required for activity.

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For research use only, not for clinical use.