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| Product Name | Purine Nucleoside Phosphorylase (PNP) |
| Catalog No. | IEC-HMM-0132 |
| Description | Alta DiagnoTech Purine Nucleoside Phosphorylase (PNP) is a microorganism-derived enzyme supplied as white amorphous lyophilized powder. The enzyme has a molecular weight of 35 kDa (SDS-PAGE) by SDS-PAGE. |
| Intended Use | Enzyme reagent for enzymatic cycling assay development and biochemical analysis involving purine nucleoside phosphorylase (pnp). |
| Principle / Technology | One unit causes the formation of 1 µmol of uric acid per minute at pH 7.7 and 37°C. |
| Detection Method | Enzymatic cycling assay |
| Performance Range / Specifications | EC 2.4.2.1; CAS 9030-21-1; Activity: ≥80 U/mg solid; Specific activity: ≥190 U/mg protein |
| Reaction Conditions / Protocol | Optimum pH: 8.0-8.5; Optimum temperature: 55°C |
| Components / Formulation | White amorphous lyophilized powder |
| Storage Conditions | Aliquot and store at or below -20°C. |
| Shelf Life | 18 months at -20°C. |
| Product Form | White amorphous lyophilized powder |
| Key Features | White amorphous lyophilized powder; Molecular weight: 35 kDa (SDS-PAGE); EC 2.4.2.1; Specific activity: ≥190 U/mg protein; Optimum pH: 8.0-8.5 |
| Activity / Unit Definition | Activity: ≥80 U/mg solid; Specific activity: ≥190 U/mg protein; Unit definition: One unit causes the formation of 1 µmol of uric acid per minute at pH 7.7 and 37°C. |
| Molecular Weight | 35 kDa (SDS-PAGE) |
| Source / Origin | Microorganism-derived |
| pH Range / Optimal pH | 8.0-8.5 |
| Application Notes / Precautions | Aliquot before storage. |
| Alternative Names / Synonyms | Purine Nucleoside Phosphorylase (PNP); PNP |
For research use only, not for clinical use.
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