Purine Nucleoside Phosphorylase PNP Enzyme >=12 U/mg 45 kDa Isoelectric Point 5.0 CAS 9030-21-1

Name: Purine Nucleoside Phosphorylase PNP Enzyme >=12 U/mg 45 kDa Isoelectric Point 5.0 CAS 9030-21-1
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Cat.No: IEC-HMM-0281 Datasheet

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Product Name	Purine Nucleoside Phosphorylase PNP Enzyme >=12 U/mg 45 kDa Isoelectric Point 5.0 CAS 9030-21-1
Catalog No.	IEC-HMM-0281
Description	Purine nucleoside phosphorylase from Alta DiagnoTech catalyzes the phosphorolysis of purine nucleosides to free bases and ribose-1-phosphate. It is widely distributed in tissues and plays a role in nucleotide metabolism and related diagnostic assays.
Intended Use	Enzyme raw material for nucleoside metabolism assays and coupled diagnostic reagents.
Principle / Technology	Catalyzes the reversible phosphorolysis of purine nucleosides such as inosine, generating hypoxanthine and ribose-1-phosphate.
Detection Method	Coupled spectrophotometric or HPLC-based assays.
Sample Type	Serum, plasma, cell lysates.
Performance Range / Specifications	Specific activity >=12.0 U/mg. Molecular weight approximately 45 kDa. Isoelectric point 5.0.
Components / Formulation	Purine nucleoside phosphorylase lyophilized powder.
Storage Conditions	-20°C.
Shelf Life	Stable for at least 12 months at -20°C.
Package Specifications	1 g per bottle; KU units available.
Product Form	White to pale yellow amorphous powder.
Activity / Unit Definition	>=12.0 U/mg
Molecular Weight	Approximately 45 kDa
Source / Origin	Microbial fermentation.
pH Range / Optimal pH	Optimal pH 6.0; stable between pH 5.0-10.0.
Shipping Conditions	Cold chain shipment.
Expiration Date / Stability	-20°C stable for one year.
Appearance	White to pale yellow amorphous powder.
CAS Number	9030-21-1
Isoelectric Point	5.0
Inhibitors	Not inhibited by sodium azide.
Optimal Temperature	>45°C.
Thermal Stability	Stable below 70°C.
Handling Precaution	Store desiccated; protect from moisture.
Michaelis Constant	6.4×10^-5 M (Inosine); 3.2×10^-4 M (Pi)

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For research use only, not for clinical use.