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| Product Name | Primer/Probe-Premix-Compatible Hot-Start Taq DNA Polymerase System |
| Catalog No. | IEC-HMM-0092 |
| Description | A flexible hot-start Taq polymerase system with strong 3′ mismatch recognition, high-specificity antibody blocking and optional dUTP/UDG carryover control. |
| Intended Use | Hot-start PCR/qPCR; gene-expression analysis; multiplex pathogen detection; tumor-target testing. |
| Principle / Technology | A directionally selected Taq enzyme is tightly blocked by dual-species antibodies at 55°C. Separate enzyme and buffer formats allow adjustment of enzyme, Mg²⁺ and nucleotide concentrations, while thermolabile UDG and uracil-containing nucleotides support carryover control. |
| Detection Method | PCR or fluorescent qPCR, depending on assay design. |
| Sample Type | DNA or cDNA templates used in singleplex and multiplex amplification assays. |
| Performance Range / Specifications | 200 or 500 reactions; primer/probe premixes remained stable after 28 days at 37°C and 50 freeze–thaw cycles in the reported accelerated evaluation. |
| Specificity | Strong 3′-end mismatch recognition and tight hot-start blocking reduce nonspecific amplification. |
| Reaction Conditions / Protocol | Formulate the reaction using the separated enzyme and buffer components. Adjust enzyme, Mg²⁺ and nucleotide concentrations as required, and include thermolabile UDG/dUTP when carryover control is needed. |
| Components / Formulation | Hot-start Taq DNA polymerase and reaction buffer supplied separately, plus thermolabile UDG, dUTP and dTTP components. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 200 reactions; 500 reactions. |
| Product Form | Modular hot-start Taq DNA polymerase system. |
| Quality Control | Primer/probe premix stability, amplification linearity, sensitivity and carryover-control performance are evaluated. |
| Key Features | Stable full primer/probe premixing; strong mismatch recognition; flexible enzyme/buffer adjustment; thermolabile UDG option; multiplex compatibility. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Suitable for hot-start PCR and qPCR systems requiring custom enzyme, Mg²⁺ or dNTP concentrations. |
For research use only, not for clinical use.
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