PNGase F-Fast for Rapid Antibody N-Glycan Release in Minutes

Name: PNGase F-Fast for Rapid Antibody N-Glycan Release in Minutes
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Cat.No: IEC-HMM-0240 Datasheet

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Product Name	PNGase F-Fast for Rapid Antibody N-Glycan Release in Minutes
Catalog No.	IEC-HMM-0240
Description	Alta DiagnoTech PNGase F-Fast is an amidase reagent for rapid removal of high-mannose, hybrid and complex N-linked glycans from glycoproteins while retaining the released N-glycans intact.
Intended Use	Rapid deglycosylation of glycosylated antibodies and fusion antibodies; purified released glycans can be used for downstream mass-spectrometric glycoform identification.
Principle / Technology	Cleaves the glycosidic linkage between the innermost N-acetylglucosamine and asparagine of Asn-linked N-glycans, enabling rapid, non-preferential release for chromatography or mass-spectrometry workflows.
Performance Range / Specifications	No detectable exoglycosidase, endoglycosidase F1, F2, F3 or H, and no protease activity.
Specificity	Hydrolyzes Asn-linked N-glycans while preserving released N-glycans intact; fully hydrolyzes glycans from glycosylated antibodies.
Reaction Conditions / Protocol	For heat inactivation, incubate a 20 uL reaction containing 1 uL enzyme at 75 degrees C for 10 minutes.
Components / Formulation	PNGase F-Fast, 50 uL; 5x PNGase F-Fast buffer, 1,000 uL.
Storage Conditions	Store at -25 to -15 degrees C
Package Specifications	50 uL enzyme with 1,000 uL of 5x buffer
Product Form	Colorless liquid
Molecular Weight	35 kDa by SDS-PAGE
Source / Origin	Microbial source
pH Range / Optimal pH	Optimum pH 7.0-8.0
Appearance	Colorless liquid
Alias	PNGase F-Fast
Product Type	Rapid peptide-N-glycosidase reagent
Function / Efficacy	Release intact N-glycans from glycoproteins for downstream glycoform analysis.
EC Number	EC 3.5.1.52
Isoelectric Point	8.14
Inhibitors	SDS
Optimal Temperature	65 degrees C
Activator	DTT

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For research use only, not for clinical use.