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| Product Name | Non-Liposomal Lipid Nanoparticle Transfection Reagent for mRNA Delivery |
| Catalog No. | ATR-CTR-0018 |
| Description | A non-liposomal lipid nanoparticle formulation specifically engineered for efficient intracellular delivery of messenger RNA. The ionizable lipid component facilitates endosomal escape at acidic pH, enabling high mRNA translation efficiency with minimal cytotoxicity. The nanoparticle system self-assembles with mRNA upon simple mixing, forming uniform particles suitable for both in vitro cell transfection and in vivo applications including vaccine development and protein replacement therapy research. |
| Intended Use | Delivery of messenger RNA into mammalian cells for transient protein expression, vaccine development, and cell reprogramming applications. |
| Principle / Technology | Ionizable lipid nanoparticle self-assembly with mRNA cargo and pH-dependent endosomal escape |
| Detection Method | Fluorescence microscopy or flow cytometry for reporter mRNA; ELISA or Western blot for protein expression quantification |
| Sample Type | HEK293, HeLa, primary fibroblasts, dendritic cells, T cells, and other mammalian cell types |
| Performance Range / Specifications | Transfection efficiency: 70–95% for mRNA encoding GFP in HEK293 cells; particle size: 80–120 nm |
| Sensitivity / LOD | Detectable protein expression from as little as 50 ng mRNA per 10⁵ cells |
| Specificity | Optimized for mRNA; DNA transfection possible but with lower efficiency than dedicated DNA transfection reagents |
| Reaction Conditions / Protocol | Mix mRNA with lipid nanoparticle reagent at 4:1 (v/w) ratio in acidic buffer; incubate 15 min at room temperature for nanoparticle formation; add to cells in complete medium; expression detectable within 2–6 hr |
| Components / Formulation | Ionizable lipid mixture in ethanol, acidic assembly buffer, neutralization buffer, fluorescent control mRNA (eGFP) |
| Storage Conditions | 2–8 °C for lipid reagent; –80 °C for control mRNA |
| Shelf Life | 6 months for lipid reagent at 2–8 °C; 12 months for control mRNA at –80 °C |
| Package Specifications | 50 transfections (24-well plate format, 0.5 µg mRNA per well) |
| Product Form | Liquid lipid mixture in ethanol; lyophilized control mRNA |
| Quality Control | Each lot validated by eGFP mRNA transfection in HEK293 cells; transfection efficiency and viability compared to reference lot |
| Key Features | Ionizable lipid chemistry provides efficient endosomal escape without the cytotoxicity associated with permanently cationic lipids |
| Purity | Endotoxin-free; sterile-filtered; certified nuclease-free |
| Concentration | As specified (typically 1 mg/mL for lipid reagents) |
| Activity / Unit Definition | Transfection efficiency: ≥70% in HEK293 cells with control plasmid at recommended ratio |
| Molecular Weight | Varies by formulation as specified in product documentation |
| Source / Origin | Synthetic cationic lipids and polymers; recombinant viral components where applicable |
| pH Range / Optimal pH | Lipoplex formation optimal at pH 7.0–7.4 |
| Shipping Conditions | Cold packs; some formulations require –20 °C; do not freeze lipid emulsions |
| Expiration Date / Stability | 6–12 months at recommended storage temperature |
| Regulatory / Compliance | Research use only; biosafety level 1 for standard transfection; BSL-2 for viral systems |
| Compatibility | Compatible with serum-containing and serum-free culture media; check product-specific compatibility |
| Recommended Buffer System | Serum-free medium or PBS for complex formation; complete medium for post-transfection culture |
| Application Notes / Precautions | Optimize reagent-to-DNA ratio per cell type; avoid antibiotic during transfection; monitor cytotoxicity |
| Batch-to-Batch Consistency | Transfection efficiency within ±15% of reference lot on HEK293 standard |
For research use only, not for clinical use.
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