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| Product Name | Dendrimer-Based Transfection Reagent, Activated Polyamidoamine |
| Catalog No. | ATR-CTR-0016 |
| Description | An activated polyamidoamine dendrimer transfection reagent with a highly branched spherical architecture providing multiple DNA-binding sites for efficient gene delivery in a defined, non-lipid chemical delivery system. |
| Intended Use | Transient and stable transfection of plasmid DNA into a broad range of mammalian cell types; particularly useful for cells sensitive to lipid-based reagents or where lipid-induced gene expression artifacts must be avoided. |
| Principle / Technology | Activated PAMAM dendrimers (generation 5–7) possess a high density of terminal amine groups for DNA binding and a defined molecular architecture; the spherical shape provides a consistent compact structure for reproducible transfection; the proton-sponge effect of internal tertiary amines facilitates endosomal escape. |
| Detection Method | Reporter gene expression by luciferase assay, cell viability by MTT or metabolic assay, gene expression profiling to detect lipid-induced artifacts |
| Sample Type | Broad range of mammalian cell lines, primary cells, cells sensitive to lipid-based transfection reagents |
| Performance Range / Specifications | Transfection efficiency 30–70% for standard cell lines; 20–50% for primary cells; cell viability >80% at optimal ratio |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Dilute DNA in serum-free medium; activate dendrimer per protocol; mix activated dendrimer with DNA; incubate 15 min; add to cells; replace medium after 4–24 hours; assay 24–72 hours. |
| Components / Formulation | Activated PAMAM dendrimer solution in methanol, sterile water for reconstitution, detailed activation protocol |
| Storage Conditions | 2–8°C, protect from light and moisture |
| Shelf Life | 12 months |
| Package Specifications | 100 transfections (24-well), 500 transfections |
| Product Form | Liquid concentrate requiring activation |
| Quality Control | Dendrimer generation and purity by MALDI-TOF, transfection efficiency validation with reference cell panel, cytotoxicity, endotoxin |
| Key Features | Defined chemical structure; non-lipid chemistry; consistent particle size; minimal gene expression artifacts; broad cell compatibility; activated form for immediate use |
For research use only, not for clinical use.
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