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| Product Name | Electroporation Cuvette Set with Buffer, 0.2 cm Gap |
| Catalog No. | ATR-CTR-0005 |
| Description | A complete electroporation system component set including sterile cuvettes with 0.2 cm electrode gap and optimized electroporation buffer for the delivery of nucleic acids into mammalian cells by high-voltage electrical pulse. |
| Intended Use | Physical delivery of plasmid DNA, siRNA, mRNA, and CRISPR RNP complexes into mammalian cells through transient membrane pore formation induced by controlled electrical pulses. |
| Principle / Technology | Brief high-voltage electric pulse temporarily disrupts the lipid bilayer of the cell membrane, creating transient aqueous pores through which nucleic acids diffuse into the cytoplasm; membrane reseals within minutes after pulse, trapping cargo inside. |
| Detection Method | Reporter gene expression (GFP), cell viability by trypan blue or flow cytometry 24 hours post-electroporation |
| Sample Type | All mammalian cell types: cell lines, primary cells, stem cells, immune cells |
| Performance Range / Specifications | Transfection efficiency 50–95% depending on cell type and pulse parameters; viable cell recovery 50–90% |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Resuspend cells in electroporation buffer at 1–5 × 10^7 cells/mL; mix with DNA/RNA; transfer to cuvette; pulse using optimized parameters; immediately add pre-warmed complete medium; transfer to culture vessel. |
| Components / Formulation | Electroporation cuvettes (0.2 cm gap, sterile, pack of 50), electroporation buffer (ATP- and glutathione-supplemented, sterile, 50 mL) |
| Storage Conditions | 15–30°C; buffer at 2–8°C |
| Shelf Life | 24 months (cuvettes); 12 months (buffer) |
| Package Specifications | 50 cuvettes + 50 mL buffer per kit |
| Product Form | Sterile cuvettes and liquid buffer |
| Quality Control | Cuvette electrical resistance consistency, buffer sterility and pH, electroporation validation with HEK293 standard |
| Key Features | Complete starter set; sterile cuvettes; optimized buffer preserves viability; validated for CRISPR RNP delivery; compatible with major electroporators |
For research use only, not for clinical use.
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