- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | DEAE-Dextran Transfection Reagent, High Molecular Weight |
| Catalog No. | ATR-CTR-0015 |
| Description | A high-molecular-weight diethylaminoethyl-dextran polymer solution for the transient transfection of mammalian cells through a simple and economical chemical method, historically significant and still used for specific applications. |
| Intended Use | Transient transfection of COS cells and other cell lines for recombinant protein production; transient expression in BHK, CV-1, and other cell lines requiring high plasmid copy number per cell. |
| Principle / Technology | Positively charged DEAE-dextran polymer complexes negatively charged DNA and binds to the cell surface via electrostatic interactions; treatment with chloroquine or DMSO shock enhances endosomal release and DNA delivery to the nucleus. |
| Detection Method | Reporter gene expression by enzymatic assay or flow cytometry, transient protein production measurement |
| Sample Type | COS-1, COS-7, CV-1, BHK-21, and other cell lines susceptible to DEAE-dextran transfection |
| Performance Range / Specifications | Transient expression in 30–80% of cells depending on cell line and protocol; high plasmid copy number per cell achieved |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Mix DNA with DEAE-dextran in serum-free medium; incubate with cells for 30 min to 4 hours; remove DNA-DEAE mixture; apply DMSO or chloroquine shock (optional); add complete medium; assay 48–72 hours post-transfection. |
| Components / Formulation | DEAE-dextran hydrochloride (average MW 500,000), lyophilized or in sterile water at 10 mg/mL |
| Storage Conditions | 15–30°C (dry powder); 2–8°C (solution); protect from moisture |
| Shelf Life | 36 months (dry); 12 months (solution) |
| Package Specifications | 1 g (powder), 10 mL (10 mg/mL sterile solution) |
| Product Form | Lyophilized powder or sterile-filtered liquid |
| Quality Control | Molecular weight by GPC, transfection validation with COS-7 reference cells, endotoxin <0.1 EU/mg, sterility |
| Key Features | Cost effective; high plasmid copy number; established protocol; suitable for COS cell expression; economical for large-scale transient production |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
