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| Product Name | siRNA Transfection Reagent, Cationic Lipid-Free, Low Cytotoxicity |
| Catalog No. | ATR-CTR-0025 |
| Description | Lipid-free siRNA delivery reagent using amphipathic peptide carrier for efficient cytoplasmic siRNA delivery with minimal cytotoxicity. Achieves >90% target gene knockdown while maintaining superior cell health compared to cationic lipid reagents. Ideal for long-term gene silencing, primary cell transfection, and genome-wide siRNA library screening. |
| Intended Use | siRNA-mediated gene knockdown; genome-wide siRNA screening; long-term silencing studies (>7 days); co-transfection with plasmid DNA; primary and difficult-to-transfect cell delivery. |
| Principle / Technology | Amphipathic peptide forms nanoparticles with siRNA; internalized via endocytosis; endosomolytic domain facilitates cytoplasmic release for RISC loading |
| Detection Method | For 24-well: dilute 10-30 pmol siRNA + 1-3 µL reagent in serum-free medium; complex 15-20 min; add to cells; incubate 24-72 hr; assess KD by qPCR/Western |
| Sample Type | Adherent and suspension cells; primary cells (HUVEC, hepatocytes, keratinocytes, macrophages) |
| Sensitivity / LOD | Effective at 1-30 nM siRNA; >90% KD for highly expressed genes |
| Reaction Conditions / Protocol | Complex: 15-20 min RT; transfection: 24-72 hr; KD detectable at 24 hr, maximal at 48-72 hr |
| Components / Formulation | Amphipathic peptide carrier (1 mg/mL), siRNA dilution buffer, GAPDH control siRNA, negative control siRNA, Cy3-siRNA fluorescent control |
| Storage Conditions | -20 °C; peptide carrier at 4 °C after thaw |
| Shelf Life | 12 months at -20 °C; peptide carrier 3 months at 4 °C after thaw |
| Package Specifications | 0.5 mL, 1 mL |
| Product Form | Sterile peptide solution; lyophilized control siRNAs |
| Key Features | Lipid-free — significantly lower cytotoxicity; >90% KD; effective in primary/difficult cells; includes controls; serum-compatible up to 10% FBS |
| Purity | Peptide >95% HPLC; endotoxin <0.1 EU/mg; control siRNAs HPLC-purified >90% duplex |
| Concentration | As specified (typically 1 mg/mL for lipid reagents) |
| Activity / Unit Definition | Transfection efficiency: ≥70% in HEK293 cells with control plasmid at recommended ratio |
| Molecular Weight | Varies by formulation as specified in product documentation |
| Source / Origin | Synthetic cationic lipids and polymers; recombinant viral components where applicable |
| pH Range / Optimal pH | Lipoplex formation optimal at pH 7.0–7.4 |
| Shipping Conditions | Cold pack -20 °C; peptide carrier maintain <4 °C |
| Expiration Date / Stability | 12 months at -20 °C; peptide carrier 3 months at 4 °C; do not refreeze; siRNAs 6 months at -20 °C |
| Regulatory / Compliance | For laboratory and research use only; RUO; ISO 9001 |
| Compatibility | Compatible with serum-containing and serum-free culture media; check product-specific compatibility |
| Recommended Buffer System | Serum-free medium or PBS for complex formation; complete medium for post-transfection culture |
| Application Notes / Precautions | Optimize reagent-to-DNA ratio per cell type; avoid antibiotic during transfection; monitor cytotoxicity |
| Batch-to-Batch Consistency | Transfection efficiency within ±15% of reference lot on HEK293 standard |
For research use only, not for clinical use.
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