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| Product Name | BacMam Transduction Reagent System for Mammalian Cells |
| Catalog No. | ATR-CTR-0019 |
| Description | A baculovirus-based gene delivery system utilizing a modified Autographa californica nuclear polyhedrosis virus engineered to express mammalian cell-active promoters. The BacMam system delivers genes of up to 38 kb into dividing and non-dividing mammalian cells without viral replication. This transduction-based approach provides highly uniform gene expression across the cell population with minimal cytotoxicity, making it suitable for high-throughput screening assay development. |
| Intended Use | Baculovirus-mediated delivery of large transgenes into mammalian cells for stable and transient expression in screening applications. |
| Principle / Technology | Modified baculovirus transduction with mammalian promoter-driven transgene expression |
| Detection Method | Fluorescence microscopy, luminescence plate reader, or flow cytometry depending on reporter construct |
| Sample Type | HEK293, HeLa, U2OS, CHO, primary hepatocytes, neural stem cells, and iPSC-derived cells |
| Performance Range / Specifications | Transduction efficiency: 80–95% in HEK293 cells at MOI 50–100; cargo capacity: up to 38 kb |
| Sensitivity / LOD | Reproducible expression in non-dividing primary cells that are refractory to plasmid transfection |
| Specificity | Broad cell type tropism; virus does not replicate in mammalian cells, classified at biosafety level 1 |
| Reaction Conditions / Protocol | Dilute baculovirus stock in culture medium to desired MOI; add to cells; incubate 4–6 hr; replace with fresh medium; transgene expression detectable at 16–24 hr post-transduction |
| Components / Formulation | BacMam viral particles (ready-to-use), BacMam enhancer solution (sodium butyrate or trichostatin A), positive control BacMam-GFP virus |
| Storage Conditions | Viral particles: 2–8 °C for short-term (2 weeks) or –80 °C for long-term; enhancer: –20 °C |
| Shelf Life | 12 months at –80 °C for virus; 24 months for enhancer |
| Package Specifications | 1 mL concentrated virus stock (approximately 10⁸ infectious units/mL) |
| Product Form | Liquid viral suspension |
| Quality Control | Infectious titer determined by GFP expression in HEK293 cells; sterility verified |
| Key Features | Large cargo capacity accommodates full-length genes and multi-gene cassettes that exceed AAV and lentivirus packaging limits |
| Purity | Endotoxin-free; sterile-filtered; certified nuclease-free |
| Concentration | As specified (typically 1 mg/mL for lipid reagents) |
| Activity / Unit Definition | Transfection efficiency: ≥70% in HEK293 cells with control plasmid at recommended ratio |
| Molecular Weight | Varies by formulation as specified in product documentation |
| Source / Origin | Synthetic cationic lipids and polymers; recombinant viral components where applicable |
| pH Range / Optimal pH | Lipoplex formation optimal at pH 7.0–7.4 |
| Shipping Conditions | Cold packs; some formulations require –20 °C; do not freeze lipid emulsions |
| Expiration Date / Stability | 6–12 months at recommended storage temperature |
| Regulatory / Compliance | Research use only; biosafety level 1 for standard transfection; BSL-2 for viral systems |
| Compatibility | Compatible with serum-containing and serum-free culture media; check product-specific compatibility |
| Recommended Buffer System | Serum-free medium or PBS for complex formation; complete medium for post-transfection culture |
| Application Notes / Precautions | Optimize reagent-to-DNA ratio per cell type; avoid antibiotic during transfection; monitor cytotoxicity |
| Batch-to-Batch Consistency | Transfection efficiency within ±15% of reference lot on HEK293 standard |
For research use only, not for clinical use.
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