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| Product Name | Nanoparticle Tracking and Uptake Assay Kit with Fluorescent DNA |
| Catalog No. | ATR-CTR-0017 |
| Description | A specialized kit containing Cy3-labeled plasmid DNA, optimized transfection reagent, and cellular uptake assay components for investigating and optimizing nucleic acid delivery mechanisms and intracellular trafficking in live cells. |
| Intended Use | Optimizing transfection conditions for new cell types; studying cellular uptake mechanisms (endocytosis pathway analysis); comparing transfection reagent performance by quantitative intracellular DNA tracking; live-cell imaging of DNA delivery. |
| Principle / Technology | Cy3 fluorophore covalently attached to plasmid DNA enables fluorescent tracking of DNA-lipid complexes during cellular uptake, endosomal trafficking, and nuclear import; co-staining with organelle-specific dyes reveals intracellular localization and delivery bottlenecks. |
| Detection Method | Confocal fluorescence microscopy for subcellular localization, flow cytometry for quantitative uptake measurement, co-localization analysis with endosome/lysosome/nuclear markers |
| Sample Type | Any mammalian cell type for transfection optimization and uptake mechanism studies |
| Performance Range / Specifications | Cy3 labeling density optimized for fluorescence detection without affecting transfection efficiency; >95% labeling efficiency; DNA complex formation comparable to unlabeled DNA |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Use Cy3-labeled DNA alone or mixed with unlabeled DNA (e.g., 1:10 ratio); transfect using standard protocol; perform live-cell or fixed-cell imaging with organelle markers; quantify uptake by flow cytometry or image analysis. |
| Components / Formulation | Cy3-labeled control plasmid DNA (5 µg), Cy3 labeling kit, transfection reagent, LysoTracker dye for lysosome staining, Hoechst nuclear stain, detailed imaging protocol |
| Storage Conditions | -20°C (labeled DNA, protect from light); 2–8°C (reagent); 15–30°C (stains) |
| Shelf Life | 12 months at -20°C (labeled DNA); use stains within 3 months |
| Package Specifications | Kit for 50 imaging experiments (24-well coverslip format) |
| Product Form | Labeled DNA, reagent, and fluorescent dyes in kit format |
| Quality Control | DNA labeling density, fluorescence intensity, transfection efficiency comparison with unlabeled DNA, photostability, DNase/RNase free |
| Key Features | Fluorescent DNA for live tracking; quantitative uptake measurement; includes organelle markers; validated for confocal microscopy; batch-to-batch labeling consistency |
For research use only, not for clinical use.
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