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| Product Name | Calcium Phosphate Co-Precipitation Transfection Kit |
| Catalog No. | ATR-CTR-0004 |
| Description | A complete kit for calcium phosphate-mediated DNA transfection, one of the earliest and most widely used chemical transfection methods, providing reproducible results for HEK293 and other cell lines at low cost. |
| Intended Use | Transient transfection of adherent cell lines for lentivirus and retrovirus production; stable cell line generation; and general gene expression studies where economical, large-scale transfection is needed. |
| Principle / Technology | DNA is co-precipitated with calcium phosphate to form fine crystalline particles that adhere to the cell surface and are taken up by endocytosis or phagocytosis; the precipitate gradually dissolves in the acidic endosomal environment, releasing the DNA payload into the cytoplasm. |
| Detection Method | GFP reporter fluorescence by flow cytometry, viral titer measurement by functional assay |
| Sample Type | HEK293T, HEK293, HeLa, NIH/3T3, COS-7, and other adherent lines susceptible to calcium phosphate transfection |
| Performance Range / Specifications | Transfection efficiency 40–80% for HEK293T; lentiviral titer 10^6–10^7 TU/mL from standard packaging protocol |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Mix DNA with calcium chloride; add dropwise to HEPES-buffered saline with gentle bubbling; incubate 15–20 min for precipitate formation; add to cells; incubate 4–16 hours; replace medium; analyze 48–72 hours post-transfection. |
| Components / Formulation | 2.5 M Calcium Chloride solution, 2x HEPES-Buffered Saline (pH 7.05), sterile water, detailed protocol |
| Storage Conditions | 15–30°C; 2–8°C after opening for extended stability |
| Shelf Life | 12 months |
| Package Specifications | Kit for 200 transfections (10 cm dish scale), 500 transfections |
| Product Form | Liquid reagents in kit format |
| Quality Control | HEPES buffer pH ±0.02, transfection efficiency validation with HEK293T, sterility |
| Key Features | Complete kit; economical; standard for lentivirus production; reproducible results; established protocol; large-scale capable |
For research use only, not for clinical use.
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