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| Product Name | Lentiviral Packaging Plasmid System, Second Generation |
| Catalog No. | ATR-CTR-0009 |
| Description | A second-generation lentivirus packaging system consisting of three plasmids (transfer vector with transgene, packaging plasmid psPAX2, envelope plasmid pMD2.G) for the production of replication-incompetent lentiviral particles in HEK293T cells. |
| Intended Use | Production of high-titer, VSV-G pseudotyped lentiviral particles for stable gene delivery into dividing and non-dividing mammalian cells, suitable for gene overexpression, shRNA knockdown, and CRISPR sgRNA library delivery. |
| Principle / Technology | The viral packaging genes (gag, pol, rev, tat) are separated onto the packaging plasmid, while VSV-G envelope is provided on a separate plasmid, increasing biosafety; the transfer plasmid contains only the transgene flanked by LTR sequences, ensuring replication incompetence of packaged virions. |
| Detection Method | Lentiviral titer by p24 ELISA, functional titer by antibiotic resistance colony formation or GFP expression in target cells |
| Sample Type | Target mammalian cells: dividing and non-dividing cell lines, primary neurons, stem cells, tissues in vivo |
| Performance Range / Specifications | Viral titer 10^6–10^8 TU/mL before concentration; >10^9 TU/mL after ultracentrifugation or PEG precipitation concentration |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Co-transfect HEK293T cells with 3 or 4 plasmids using calcium phosphate or PEI; collect supernatant at 48 and 72 hours; filter through 0.45 µm; concentrate if needed; aliquot and store at -80°C. |
| Components / Formulation | psPAX2 packaging plasmid (gag, pol, rev, tat), pMD2.G envelope plasmid (VSV-G), control transfer plasmid (CMV-EGFP), bacterial stabs for each |
| Storage Conditions | -20°C (plasmid DNA); -80°C (virus aliquots) |
| Shelf Life | 36 months (plasmid DNA at -20°C); 12 months (virus at -80°C) |
| Package Specifications | 10 µg each plasmid, bacterial stabs |
| Product Form | Plasmid DNA in TE buffer |
| Quality Control | Plasmid sequence verification, endotoxin <0.1 EU/µg, virus titer validation by functional assay on HEK293 cells |
| Key Features | Second-generation system for enhanced biosafety; VSV-G pseudotyped for broad tropism; validates packaging efficiency; standard for lentivirus production in research laboratories |
For research use only, not for clinical use.
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