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| Product Name | Polyethylenimine (PEI) Transfection Reagent, Linear 25 kDa |
| Catalog No. | ATR-CTR-0003 |
| Description | A high-molecular weight linear polyethylenimine reagent for cost-effective large-scale transient transfection in suspension and adherent cell cultures, widely used in recombinant protein and viral vector production. |
| Intended Use | Large-scale transient gene expression in HEK293 and CHO suspension cultures for recombinant antibody, protein, and lentiviral/AAV vector production in research and bioprocessing applications. |
| Principle / Technology | Linear PEI 25 kDa contains a high density of protonatable amine groups that condense DNA into positively charged nanoparticles; after endocytosis, the proton sponge effect causes endosome swelling and rupture through osmotic imbalance, releasing DNA into the cytoplasm. |
| Detection Method | Recombinant protein yield by ELISA or activity assay, viral titer by qPCR or transduction assay |
| Sample Type | HEK293F, HEK293T, Expi293, CHO-S, CHO-K1, and other suspension-adapted cell lines |
| Performance Range / Specifications | IgG yields 100–1000 mg/L for HEK293 suspension cultures at 1 L scale; transfection efficiency >80% in adherent HEK293 |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Prepare DNA and PEI separately in 150 mM NaCl at 1:2 to 1:3 DNA:PEI ratio (w/w); mix and incubate 15–20 min; add to cells dropwise; no medium change required; harvest 3–7 days post-transfection. |
| Components / Formulation | Linear polyethylenimine 25 kDa, 1 mg/mL in water, pH 7.0, 0.2 µm filtered, endotoxin tested |
| Storage Conditions | 2–8°C or -20°C; protected from moisture; aliquot to avoid repeated freeze-thaw |
| Shelf Life | 24 months at -20°C; 6 months at 2–8°C after thawing |
| Package Specifications | 100 mg (100 mL), 1 g |
| Product Form | Liquid, filtered, endotoxin tested |
| Quality Control | Molecular weight by GPC, amine content, transfection efficiency with HEK293 standard, sterility, endotoxin <0.1 EU/µg |
| Key Features | Cost effective for large-scale production; high protein yield; validated for HEK293 and CHO; endotoxin tested; linear PEI for low cytotoxicity |
For research use only, not for clinical use.
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