- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Nicking Endonuclease Nt.BspQI (Recognition Site: 5'-GCTCTTCN-3', Top-Strand Nicking) |
| Catalog No. | IVDRM-0165 |
| Description | Nt.BspQI is a nicking endonuclease that, unlike conventional restriction endonucleases, cleaves only one strand (the top strand) of a double-stranded DNA substrate. It recognizes the sequence 5'-GCTCTTCN-3' and introduces a single-stranded nick without completely severing the DNA molecule, making it a precise tool for applications that require site-specific nicking of dsDNA. |
| Intended Use | For molecular cloning applications requiring site-specific nicking of double-stranded DNA. |
| Principle / Technology | Nicking endonuclease: unlike traditional endonucleases, it cleaves only one strand of the dsDNA substrate. Nt.BspQI nicks the top strand one nucleotide downstream of its recognition site (5'-GCTCTTCN↓-3'), producing a nick rather than a double-strand break. |
| Sample Type | Double-stranded DNA (dsDNA) substrates. |
| Performance Range / Specifications | Concentration: 10 U/µL. One unit (U) is the amount of enzyme required to completely convert 1 µg of supercoiled pUC19 DNA to the open circular form in 1 hour at 50°C in a 50 µL reaction. |
| Specificity | Recognizes 5'-GCTCTTCN-3' and nicks the top strand. Methylation effect: Dam, Dcm, CpG, EcoKI, and EcoBI methylation have no effect on cleavage. |
| Reaction Conditions / Protocol | 1× Nt.BspQI reaction buffer; incubate at 50°C. Heat inactivation: 80°C for 20 min. |
| Components / Formulation | Nt.BspQI nicking endonuclease, 10 U/µL; supplied with the recommended reaction buffer. |
| Storage Conditions | Store at -25°C to -15°C. |
| Shelf Life | 2 years when stored at -25°C to -15°C. |
| Package Specifications | 2000 U |
| Concentration | 10 U/µL |
| Activity / Unit Definition | One unit (U) is defined as the amount of enzyme required to completely convert 1 µg of supercoiled pUC19 DNA into the open circular form within 1 hour at 50°C in a 50 µL reaction. |
| Expiration Date / Stability | Stable for 2 years at -25°C to -15°C. |
| Recommended Buffer System | 1× dedicated reaction buffer (supplied). |
| Application Notes / Precautions | Incubate reactions at 50°C for optimal activity. Cleavage is not affected by Dam, Dcm, CpG, EcoKI, or EcoBI methylation. Inactivate the enzyme at 80°C for 20 min before downstream steps. Avoid repeated freeze-thaw cycles; store at -25°C to -15°C. |
| Recognition Site | 5'-GCTCTTCN↓-3' 3'-CGAGAAGN-5' (Nt.BspQI cleaves the top strand one nucleotide downstream of the recognition site, as indicated by ↓.) |
| Heat Inactivation | 80°C for 20 min. |
| Methylation Sensitivity | Dam, Dcm, CpG, EcoKI, EcoBI: no effect. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
