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| Product Name | NADP+ Sodium Salt (β-Nicotinamide Adenine Dinucleotide Phosphate), ≥98% |
| Catalog No. | IEC-HMM-0075 |
| Description | β-Nicotinamide adenine dinucleotide phosphate (NADP+) sodium salt, oxidized form, with purity ≥98% by enzymatic and spectrophotometric assays. NADP+ is the essential phosphorylated coenzyme for anabolic redox reactions catalyzed by dehydrogenases and reductases in the pentose phosphate pathway, fatty acid synthesis, and cytochrome P450 systems. Supplied as high-purity lyophilized powder for biochemical, enzymatic, and cell-based research. |
| Intended Use | Cofactor for NADP+-dependent dehydrogenase and reductase enzyme assays; glucose-6-phosphate dehydrogenase (G6PDH) activity assays; cytochrome P450 enzyme reconstitution; NADPH-regenerating system component; metabolic pathway studies. |
| Principle / Technology | NADP+ serves as a hydride (H⁻) acceptor in biological oxidations; reduced to NADPH by dehydrogenase enzymes; NADPH is the primary reductant for biosynthetic reactions (fatty acids, cholesterol, nucleotides) and antioxidant systems (glutathione reductase, thioredoxin reductase) |
| Detection Method | Prepare 10-100 mM stock solutions in water or buffer pH 7-8; NADP+ is hygroscopic — prepare solutions immediately after opening; stable in neutral to slightly alkaline solutions; avoid acidic conditions (pH <5 causes rapid decomposition) |
| Components / Formulation | β-NADP+ sodium salt hydrate (C₂₁H₂₇N₇O₁₇P₃·xNa), ≥98% purity by enzymatic assay with G6PDH |
| Storage Conditions | -20 °C, desiccated, protected from light and moisture; highly hygroscopic |
| Shelf Life | 24 months at -20 °C in sealed, desiccated container; 12 months at 4 °C |
| Package Specifications | 100 mg, 500 mg, 1 g, 5 g, 10 g in amber glass vials with desiccant |
| Product Form | Lyophilized white to off-white powder |
| Key Features | Enzymatically assayed purity ≥98%; A260/A340 ratio <2.4 (indicating low NADPH contamination); low ethanol content (<1%) — no alcohol dehydrogenase interference; low metal content for enzymatic applications; endotoxin tested for cell-based assays |
| Purity | ≥98% by G6PDH enzymatic assay; <0.5% NADPH content; ethanol <1%; water <5% by Karl Fischer; heavy metals <10 ppm |
| Concentration | Enzymes at specified U/µL; coenzymes at specified mM concentration |
| Activity / Unit Definition | One unit incorporates 10 nmol dNTP into acid-insoluble form in 30 min at specified temperature (polymerases); other enzyme units per product specification |
| Molecular Weight | 765.39 g/mol (free acid); sodium salt molecular weight varies by sodium content (typically 787-820 g/mol) |
| Source / Origin | Synthetic; enzymatic conversion of NAD+ or chemical phosphorylation |
| pH Range / Optimal pH | pH 7.5–8.5 for polymerase activity; pH 7.5–8.0 for ligation; pH 7.5–8.0 for transcription |
| Shipping Conditions | Ambient temperature; protect from light and moisture |
| Expiration Date / Stability | 24 months at -20 °C desiccated, protected from light; 12 months at 4 °C; stock solutions: 2 weeks at 4 °C pH 7-8, 3 months at -20 °C (aliquot); acidic solutions decompose within hours — never store NADP+ below pH 5 |
| Regulatory / Compliance | For laboratory and research use only; RUO; CAS 1184-16-3 (free acid); not classified as dangerous goods; ISO 9001 |
| Compatibility | Soluble in water (>100 mg/mL); dissolve in PBS or Tris buffer pH 7-8; NADP+ decomposes in acidic conditions (pH <5); avoid solutions containing cyanide, bisulfite, or reducing agents which react with the nicotinamide ring; spectrophotometric monitoring at 340 nm for NADPH formation |
| Recommended Buffer System | Tris-HCl, KCl, MgCl₂-based buffers; glycerol-containing storage buffers |
| Application Notes / Precautions | Aliquot enzymes to avoid repeated freeze-thaw; use dedicated nuclease-free workspace; verify enzyme activity for critical applications |
| Batch-to-Batch Consistency | Enzyme specific activity within ±15% of reference lot; functional testing per lot |
For research use only, not for clinical use.
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