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| Product Name | NAD+ Oxidized Form (β-Nicotinamide Adenine Dinucleotide) |
| Catalog No. | IEC-HMM-0069 |
| Description | High-purity β-nicotinamide adenine dinucleotide in its oxidized form, supplied as a lyophilized powder or pre-dissolved solution for use as an enzyme cofactor in dehydrogenase-coupled biochemical assays. NAD+ serves as an essential electron acceptor in numerous metabolic enzyme reactions and as a substrate for enzymes including sirtuins, poly(ADP-ribose) polymerases, and ADP-ribosyltransferases. This preparation is formulated to meet the stringent purity requirements of quantitative enzymatic assays. |
| Intended Use | Enzymatic cofactor for dehydrogenase-coupled assays and substrate for NAD+-consuming enzyme activity measurements. |
| Principle / Technology | Redox cofactor cycling in dehydrogenase-coupled reaction systems |
| Detection Method | Spectrophotometric monitoring at 340 nm (NADH absorption) or coupled colorimetric/fluorometric detection |
| Sample Type | Biochemical assay systems, enzyme kinetics studies, cell-free metabolic reconstruction |
| Performance Range / Specifications | Purity: ≥98% by HPLC; ε₂₆₀ = 18.0 mM⁻¹ cm⁻¹ |
| Sensitivity / LOD | Enzymatic reduction by alcohol dehydrogenase demonstrates ≥98% functional conversion to NADH |
| Specificity | β-NAD+ selective; α-NAD+ isomer not present; free of NADH contamination as verified by A₃₄₀/A₂₆₀ ratio ≤0.05 |
| Reaction Conditions / Protocol | Prepare 100 mM stock in water or assay buffer; use at 0.1–5 mM final concentration depending on enzyme Km |
| Components / Formulation | β-NAD+ oxidized form (lyophilized powder or 100 mM aqueous solution) |
| Storage Conditions | Lyophilized powder: –20 °C desiccated; aqueous solution: –20 °C, single-use aliquots, protect from light |
| Shelf Life | 36 months for lyophilized powder; 12 months for solution at –20 °C |
| Package Specifications | 1 g, 5 g powder or 1 mL, 5 mL of 100 mM solution |
| Product Form | Lyophilized powder or frozen aqueous solution |
| Quality Control | HPLC purity ≥98%; heavy metal content ≤10 ppm; endotoxin ≤0.1 EU/mg |
| Key Features | Essential cofactor compatible with the full spectrum of NAD+/NADH-dependent enzyme assay systems |
| Purity | ≥95% by SDS-PAGE for enzymes; ≥98% by HPLC for coenzymes |
| Concentration | Enzymes at specified U/µL; coenzymes at specified mM concentration |
| Activity / Unit Definition | One unit incorporates 10 nmol dNTP into acid-insoluble form in 30 min at specified temperature (polymerases); other enzyme units per product specification |
| Molecular Weight | As specified per enzyme: Taq ~94 kDa; M-MLV RT ~75 kDa; T4 ligase ~55 kDa; coenzymes as labeled |
| Source / Origin | Recombinant, expressed in E. coli; purified by multi-step chromatography |
| pH Range / Optimal pH | pH 7.5–8.5 for polymerase activity; pH 7.5–8.0 for ligation; pH 7.5–8.0 for transcription |
| Shipping Conditions | Dry ice for enzyme shipments; cold packs for coenzyme solutions |
| Expiration Date / Stability | 24 months at –20 °C for enzymes; 12–36 months for coenzymes per product type |
| Regulatory / Compliance | Research use; ISO 9001 manufacturing; GMP-grade options available for diagnostic manufacturing |
| Compatibility | Compatible with standard PCR, RT-PCR, isothermal amplification, and molecular cloning workflows |
| Recommended Buffer System | Tris-HCl, KCl, MgCl₂-based buffers; glycerol-containing storage buffers |
| Application Notes / Precautions | Aliquot enzymes to avoid repeated freeze-thaw; use dedicated nuclease-free workspace; verify enzyme activity for critical applications |
| Batch-to-Batch Consistency | Enzyme specific activity within ±15% of reference lot; functional testing per lot |
For research use only, not for clinical use.
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