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| Product Name | N-Acetylneuraminate Lyase Enzyme 33 U per mg Specific Activity >=90% Purity 10 KU |
| Catalog No. | IEC-HMM-0270 |
| Description | N-acetylneuraminate lyase is a lyase enzyme that catalyzes the reversible cleavage of N-acetylneuraminic acid (sialic acid) into N-acetylmannosamine and pyruvate. It is supplied as a white freeze-dried powder with a specific activity of 33 U per mg of protein and a purity of at least 90% as verified by SDS-PAGE. The enzyme has an apparent molecular weight of approximately 35 kDa and is formulated in 100 mM phosphate buffer at pH 7.4. This product is intended for use as a raw material in sialic acid detection kits and diagnostic applications requiring sialic acid quantification. |
| Intended Use | For research and diagnostic manufacturing use as a raw material in sialic acid detection kits and related diagnostic applications. Not for use in human experiments. |
| Principle / Technology | Lyase-catalyzed aldol cleavage: the enzyme reversibly cleaves N-acetylneuraminic acid into N-acetylmannosamine and pyruvate, enabling sialic acid quantification in biological samples. |
| Detection Method | Enzymatic assay measuring pyruvate formation or sialic acid consumption |
| Storage Conditions | Store the freeze-dried powder at -20°C for long-term stability. |
| Shelf Life | Stable for at least 12 months when stored at -20°C. |
| Package Specifications | 10 KU per vial |
| Product Form | White freeze-dried powder |
| Key Features | Lyase enzyme catalyzing sialic acid cleavage Specific activity 33 U/mg protein Apparent molecular weight approximately 35 kDa by SDS-PAGE Purity >=90% as verified by SDS-PAGE Formulated in 100 mM phosphate buffer (pH 7.4) Suitable for sialic acid detection kit raw material |
| Purity | >=90% (SDS-PAGE) |
| Activity / Unit Definition | 33 U/mg protein |
| Molecular Weight | Approximately 35 kDa (SDS-PAGE) |
| Source / Origin | Recombinant expression |
| Recommended Buffer System | 100 mM phosphate buffer (pH 7.4) |
| Appearance | White freeze-dried powder |
| Alias | NAL; N-acetylneuraminate lyase; Sialic acid aldolase |
| CAS Number | 9027-60-5 |
| Safety | For research and manufacturing use only. Not for use in diagnostic procedures or therapeutic applications in humans. |
| Physical State | Freeze-dried powder |
| Color | White |
For research use only, not for clinical use.
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