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| Product Name | Murine RNase Inhibitor, Ultra-Clean (40 U/μL) |
| Catalog No. | IVDRM-0278 |
| Description | RNase inhibitor is a specific ribonuclease (RNase) inhibitor that inactivates RNases by forming a non-covalent complex with them. This product is a recombinant murine RNase inhibitor expressed in soluble form in E. coli and purified to high quality; it broadly inhibits various RNases, including RNase A, RNase B and RNase C. Verified by RT-PCR and RT-qPCR, it is compatible with reverse transcriptases and various DNA polymerases. Compared with the human RNase inhibitor, this murine protein lacks the two oxidation-sensitive cysteines of the human protein and therefore exhibits higher resistance to oxidation, making it more suitable for experiments sensitive to high DTT concentrations, such as qPCR. Manufactured with an ultra-clean, low nucleic acid background process, it features lower host residuals and is well suited for applications with stricter background requirements, such as pathogen detection. |
| Intended Use | Protection of RNA from RNase degradation in RT-PCR, RT-qPCR and other RNA-dependent molecular diagnostic workflows, especially pathogen detection assays requiring ultra-low nucleic acid background. |
| Principle / Technology | The inhibitor binds RNases non-covalently to form a tight complex, thereby inactivating the ribonuclease and preventing degradation of RNA templates during reverse transcription and downstream amplification. |
| Detection Method | RT-PCR, RT-qPCR |
| Performance Range / Specifications | Ultra-clean, low nucleic acid background: E. coli DNA residual < 0.1 copies per 100 U, suitable for applications with stricter background requirements Broad-spectrum RNase inhibition: inhibits RNases including RNase A, RNase B and RNase C Compatible with a wide range of reaction conditions: active from pH 5.0 to 9.0 and from 25 °C to 60 °C, suitable for thermostable reverse transcriptases (55 °C-60 °C) Compatible with a wide range of downstream experiments: no effect on the activity of SP6, T7 or T3 RNA polymerases, AMV and M-MLV reverse transcriptases, or Taq DNA polymerase Batch-to-batch stability: mature protein expression and purification platform with single-batch capacity at the tens-of-millions-of-units scale and strict quality control ensure product uniformity, stability and timely supply |
| Sensitivity / LOD | E. coli DNA residual < 0.1 copies/100 U |
| Specificity | Broadly inhibits RNase A, RNase B and RNase C. Compared with the human RNase inhibitor, this murine protein lacks the two oxidation-sensitive cysteines of the human protein, conferring higher resistance to oxidation and making it better suited for reactions with high DTT sensitivity, such as qPCR. |
| Reaction Conditions / Protocol | Active from pH 5.0 to 9.0 and from 25 °C to 60 °C; supports thermostable reverse transcriptases working at 55 °C-60 °C. |
| Storage Conditions | Store at -25 °C to -15 °C. |
| Shelf Life | 2 years |
| Package Specifications | 2 KU; 10 KU; 20 KU; 100 KU; 1,000 KU; 20,000 KU |
| Product Form | Liquid |
| Quality Control | E. coli DNA residual < 0.1 copies/100 U; batch-to-batch consistency ensured by a mature expression and purification platform with strict quality control. |
| Concentration | 40 U/μL |
| Source / Origin | Recombinant murine (mouse) RNase inhibitor expressed in soluble form in E. coli. |
| pH Range / Optimal pH | Active over pH 5.0-9.0 |
| Expiration Date / Stability | Stable for 2 years when stored at -25 °C to -15 °C. |
| Regulatory / Compliance | IVD raw material for molecular diagnostic reagent manufacturing; for research and manufacturing use only. |
| Compatibility | Compatible with reverse transcriptases (including thermostable reverse transcriptases at 55 °C-60 °C) and various DNA polymerases in RT-PCR and RT-qPCR; no effect on the activity of SP6, T7 or T3 RNA polymerases, AMV and M-MLV reverse transcriptases, or Taq DNA polymerase. |
| Batch-to-Batch Consistency | Mature protein expression and purification platform with single-batch capacity at the tens-of-millions-of-units scale; strict quality control ensures uniformity, stability and timely supply. |
For research use only, not for clinical use.
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