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Murine RNase Inhibitor

Cat.No: IEC-HMM-0104 Datasheet

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Product Name Murine RNase Inhibitor
Catalog No. IEC-HMM-0104
Description A recombinant murine RNase inhibitor with high oxidative stability and broad compatibility with reverse-transcription and amplification systems.
Intended Use First-strand cDNA synthesis; RT-PCR and qPCR; in vitro transcription or translation; RNA isolation and purification; RNase protection assays.
Principle / Technology The recombinant inhibitor binds RNase A-family enzymes and protects RNA from degradation during sample processing and enzymatic reactions.
Detection Method Used as an RNA-protection component in reverse-transcription, PCR and other RNA workflows.
Sample Type RNA samples and RNA-containing enzymatic reactions.
Performance Range / Specifications 2,000 U, 10,000 U or 20,000 U; supplied at 40 U/µL.
Specificity Broadly inhibits RNase A, RNase B and RNase C without detectable RNase contamination in the preparation.
Reaction Conditions / Protocol Add to RNA-containing reactions at an application-appropriate concentration. It can be used with commercial reverse transcriptases and DNA polymerases.
Components / Formulation Recombinant murine RNase inhibitor at 40 U/µL.
Storage Conditions Store at −20°C.
Package Specifications 2,000 U; 10,000 U; 20,000 U.
Product Form Recombinant murine RNase inhibitor solution.
Quality Control Purity, RNase contamination, inhibitory activity and compatibility with RT-PCR and RT-qPCR systems are evaluated.
Key Features High oxidative stability; inhibition of RNase A, B and C; no detectable RNase carryover; broad enzyme-system compatibility; suitable for DTT-sensitive assays.
Concentration 40 U/µL.
Source / Origin Recombinant murine RNase inhibitor expressed in soluble form in E. coli.
Compatibility Compatible with reverse transcriptases, DNA polymerases, RT-PCR and RT-qPCR systems.

For research use only, not for clinical use.

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