- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Murine RNase Inhibitor, High Concentration (200 U/μL) |
| Catalog No. | IVDRM-0197 |
| Description | Murine RNase Inhibitor (200 U/μL) is a high-concentration, recombinant form of the classic placental-type RNase inhibitor. It inactivates ribonucleases by binding non-covalently to RNase to form a stable inactive complex. Expressed and purified from E. coli in soluble form, this murine inhibitor broadly suppresses RNase A, RNase B and RNase C. Because it lacks the two oxidation-sensitive cysteines found in the human protein, it delivers superior resistance to oxidation and performs reliably in reactions sensitive to high DTT concentrations, such as qPCR. It is fully compatible with reverse transcriptases and a wide range of DNA polymerases, and is well suited to first-strand cDNA synthesis, polysome isolation, in vitro transcription and cell-free translation systems. |
| Intended Use | For protection of RNA in first-strand cDNA synthesis, polysome isolation, in vitro transcription and in vitro cell-free translation systems. |
| Principle / Technology | The inhibitor binds RNase non-covalently to form a complex that specifically inactivates the enzyme. It is a recombinant murine protein expressed in soluble form in E. coli; compared with the human inhibitor it lacks two oxidation-sensitive cysteine residues, giving higher antioxidant activity and better tolerance of high-DTT reactions. |
| Sample Type | RNA samples in enzymatic reactions (cDNA synthesis, in vitro transcription, cell-free translation, polysome isolation). |
| Performance Range / Specifications | Broad-spectrum RNase inhibition: active against RNase A, RNase B, RNase C and other RNases. Broad reaction compatibility: retains activity from pH 5.0 to 9.0 and from 25°C to 60°C, suitable for use with thermostable reverse transcriptases at 55-60°C. Broad downstream compatibility: does not affect the activity of SP6, T7 or T3 RNA polymerases, AMV or M-MLV reverse transcriptases, or Taq DNA polymerase. Validated by RT-PCR and RT-qPCR for compatibility with reverse transcriptases and various DNA polymerases. |
| Specificity | Specifically inhibits RNase A, RNase B and RNase C; no effect on SP6, T7 or T3 RNA polymerases, AMV/M-MLV reverse transcriptases, or Taq DNA polymerase. |
| Components / Formulation | Supplied in storage buffer: 20 mM Hepes-KOH, 150 mM KCl, 8 mM DTT, 0.5% NP40, 0.5% Tween-20, 50% glycerol, pH 7.5 ± 0.2 at 25°C. |
| Storage Conditions | Store at -25°C to -15°C. |
| Shelf Life | 2 years when stored at -25°C to -15°C. |
| Package Specifications | 50 μL; 1 mL; 10 mL; 50 mL; 500 mL |
| Product Form | Liquid in storage buffer (50% glycerol). |
| Concentration | 200 U/μL (high-concentration version) |
| Source / Origin | Recombinant murine RNase inhibitor gene expressed in soluble form in E. coli and purified. |
| Compatibility | Compatible with reverse transcriptases and various DNA polymerases in RT-PCR and RT-qPCR; compatible with thermostable reverse transcriptases working at 55-60°C. |
| Recommended Buffer System | 20 mM Hepes-KOH, 150 mM KCl, 8 mM DTT, 0.5% NP40, 0.5% Tween-20, 50% glycerol, pH 7.5 ± 0.2 at 25°C. |
| Batch-to-Batch Consistency | A mature protein expression and purification platform supports single-batch capacity at the tens-of-millions-of-units scale; stringent quality control ensures lot-to-lot uniformity, stability and timely supply. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
