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| Product Name | Multiplex Probe Melting-Curve Hot-Start DNA Polymerase |
| Catalog No. | IEC-HMM-0094 |
| Description | A specialized hot-start DNA polymerase for high-plex asymmetric probe PCR and multiplex melting-curve analysis, with strong mismatch recognition and inhibitor tolerance. |
| Intended Use | Multiplex pathogen detection; reproductive genetics; pharmacogenomic testing; food testing; high-plex probe melting analysis. |
| Principle / Technology | A hot-start polymerase with balanced cleavage and polymerization activities supports asymmetric probe PCR. Dual-antibody blocking, optimized buffer and dUTP/UDG carryover control improve multiplex melting-peak uniformity, specificity and robustness. |
| Detection Method | Multiplex fluorescent probe melting-curve analysis following asymmetric PCR. |
| Sample Type | DNA templates, including samples containing blood-derived inhibitors or guanidine salts. |
| Performance Range / Specifications | 500 or 2,500 U; evaluated in an eight-plex asymmetric PCR system with improved melting-peak height and uniformity. |
| Sensitivity / LOD | High amplification success in high-plex probe melting assays. |
| Specificity | Strong 3′ mismatch recognition and hot-start control reduce nonspecific melting peaks. |
| Reaction Conditions / Protocol | Formulate the asymmetric probe PCR with assay-specific primers and probes, run amplification and perform a programmed melting-curve acquisition. |
| Components / Formulation | Hot-start DNA polymerase system with optimized buffer and dUTP/UDG carryover-control chemistry. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 500 U; 2,500 U. |
| Product Form | Specialized hot-start DNA polymerase for multiplex melting analysis. |
| Quality Control | Multiplex peak height, peak uniformity, target specificity, GC compatibility and inhibitor tolerance are evaluated. |
| Key Features | High-plex capability; uniform melting peaks; strong specificity; GC-content compatibility; blood and guanidine tolerance; carryover control. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Designed for asymmetric probe-PCR platforms using multiplex melting-curve analysis. |
| Application Notes / Precautions | If melting peaks are low, optimize MgCl₂, enzyme and probe concentrations. Maintain precise pipetting and calibrated thermal control for reproducible melting analysis. |
For research use only, not for clinical use.
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