| Product Name | MTT Cell Viability and Toxicity Assay Reagent |
| Catalog No. | CATR-HMM-0015 |
| Description | MTT stands for 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, also known as thiazol blue. |
| Application | This product is widely used in cytokine activity detection, cytotoxicity detection, and many other experimental processes. |
| Applicable Instruments | Fluorescence microscope, flow cytometer |
| Storage | Store at -20°C away from light. |
| Notes | 1. The solvent may crystallize at lower temperatures; ensure it is thawed and melted before use. 2. The MTT working solution is a pale yellow solution. Do not use if the color turns black or gray-green. 3. Repeated freeze-thaw cycles may reduce detection efficacy. Although this reagent shows no significant impact on detection efficacy after three freeze-thaw cycles, it is recommended to aliquot into smaller volumes after the first thaw. During repeated freeze-thaw cycles, some precipitation may occur. Allow it to equilibrate to room temperature and dissolve as much as possible. 4. In most cases, adding the MTT working solution to cells and incubating for 4 hours yields optimal results. However, incubation time may vary depending on experimental conditions such as cell type and cell density. 5. Signal intensity and stability are affected by temperature. Ensure the MTT working solution is equilibrated to room temperature before the reaction. 6. Since 96-well plates are used for detection, evaporation issues must be addressed if cell culture duration is prolonged. On one hand, since the outer ring of the 96-well plate is most prone to evaporation, the outer ring can be discarded, and an equal volume of PBS, water, or culture medium added instead; on the other hand, the 96-well plate can be placed near a water source inside the incubator to mitigate evaporation. 7. This product is based on the succinate dehydrogenase reduction reaction. Samples should contain minimal amounts of reducing agents and antioxidants (such as reduced glutathione, mercaptoethanol, vitamin C, etc.). Before detection with an enzyme-linked immunosorbent assay (ELISA) reader, ensure that there are no bubbles in each well, as they may interfere with the measurement. |
For research use only, not for clinical use.
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