- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | LDH Release Cytotoxicity Fluorometric Assay Kit (Resazurin-Coupled) |
| Catalog No. | CATR-HMM-0053 |
| Description | A fluorometric assay kit for quantifying lactate dehydrogenase (LDH) released from damaged cells into culture supernatant. The resazurin-coupled detection system provides superior sensitivity and dynamic range compared to traditional tetrazolium salt-based LDH assays. The non-destructive sampling approach enables multiplexing with other viability assays on the same cell population, making it ideal for comprehensive cytotoxicity profiling in drug development. |
| Intended Use | Quantitative measurement of cell membrane damage and cytotoxicity; drug-induced toxicity screening; antibody-dependent cellular cytotoxicity (ADCC) assessment; complement-dependent cytotoxicity (CDC) evaluation; nanoparticle and biomaterial biocompatibility testing. |
| Principle / Technology | Released LDH catalyzes conversion of lactate to pyruvate with concomitant reduction of NAD+ to NADH; NADH drives reduction of resazurin to fluorescent resorufin via diaphorase enzyme coupling; fluorescence signal is directly proportional to LDH activity in the supernatant |
| Detection Method | Fluorometric; excitation 530–560 nm, emission 580–610 nm |
| Sample Type | Cell culture supernatant (serum-containing medium may have background LDH; use low-LDH serum or serum-free conditions for maximum sensitivity) |
| Sensitivity / LOD | Detects LDH from as few as 200 lysed cells per well; linear range 0.1–20% cytotoxicity; LOD 0.02 mU LDH per well |
| Specificity | Specific for LDH activity; no cross-reaction with other dehydrogenases; serum LDH background can be subtracted using medium-only control |
| Reaction Conditions / Protocol | Transfer 50 µL supernatant to assay plate; add 50 µL LDH detection reagent; incubate 30 minutes at room temperature protected from light; add 25 µL stop solution; read fluorescence |
| Components / Formulation | Lactate substrate solution (10×), NAD+ cofactor (lyophilized), diaphorase/resazurin detection mix (lyophilized), LDH positive control (lyophilized), lysis solution (10×), stop solution, assay buffer |
| Storage Conditions | Store at -20 °C; protect from light; avoid repeated freeze-thaw cycles |
| Shelf Life | 12 months from manufacture date when stored at -20 °C |
| Package Specifications | 200 tests, 500 tests, 1,000 tests in 96-well plate format |
| Product Form | Lyophilized reagents with liquid buffers and stop solution |
| Key Features | Fluorometric detection with 50× greater sensitivity than absorbance-based LDH assays; non-destructive sampling preserves cells for multiplex viability assays; includes positive control and lysis buffer for 100% cytotoxicity reference; compatible with high-throughput screening in 96-well and 384-well formats |
| Purity | LDH positive control specific activity >400 U/mg protein; resazurin purity >95% |
| Concentration | As specified on product label; working concentrations optimized per protocol |
| Activity / Unit Definition | Signal-to-noise ratio and linearity verified on reference cell lines per lot |
| Molecular Weight | Varies by dye or reagent component as specified |
| Source / Origin | Synthetic dyes and tetrazolium compounds; recombinant enzymes where applicable |
| pH Range / Optimal pH | pH 7.2–7.4 for cell-based assays |
| Shipping Conditions | Cold pack -20 °C; dry ice recommended for long-distance transport |
| Expiration Date / Stability | 12 months at -20 °C; reconstituted reagents stable for 2 weeks at 4 °C or 2 months at -20 °C in single-use aliquots |
| Regulatory / Compliance | For laboratory and research use only; RUO; manufactured under ISO 9001; not classified as dangerous goods |
| Compatibility | Compatible with standard cell culture media (DMEM, RPMI-1640, MEM, etc.); serum-containing media may increase background — low-LDH or heat-inactivated FBS recommended; phenol red does not interfere with fluorometric readout |
| Recommended Buffer System | PBS or phenol red-free complete medium as specified in protocol |
| Application Notes / Precautions | Include spontaneous release control (untreated cells), maximum release control (lysed cells), and medium-only background control. Calculate % cytotoxicity: (Experimental - Spontaneous) / (Maximum - Spontaneous) × 100. For time-course studies, collect supernatant aliquots at each time point and store at -80 °C; assay all samples simultaneously to minimize inter-assay variation. |
| Batch-to-Batch Consistency | Signal linearity R² ≥0.99 with reference cell number standard curve per lot |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
