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| Product Name | WST-1 Cell Proliferation Reagent |
| Catalog No. | CATR-HMM-0042 |
| Description | A water-soluble tetrazolium salt-based reagent for the colorimetric quantification of cell proliferation and viability. WST-1 produces a soluble formazan dye directly in the culture medium upon cleavage by mitochondrial succinate-tetrazolium reductase in viable cells, enabling a simple add-and-read workflow. |
| Intended Use | Measurement of cell proliferation in response to growth factors, cytokines, and mitogens; cytotoxicity screening of chemical and pharmaceutical compounds in cultured mammalian cells. |
| Principle / Technology | WST-1 (4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate) is reduced by mitochondrial succinate-tetrazolium reductase at the cell surface to yield a soluble formazan that absorbs at 440 nm; the electron coupling agent mPMS accelerates the reaction rate. |
| Detection Method | Colorimetric absorbance at 440 nm (reference 650 nm) using a microplate reader |
| Sample Type | Proliferating adherent and suspension mammalian cell lines grown in 96-well microplates with standard culture medium |
| Performance Range / Specifications | Linear range: 1,000–100,000 cells per well; incubation time 30 minutes to 4 hours depending on metabolic rate of target cells |
| Sensitivity / LOD | Approximately 1,000 viable cells per well for common adherent cell lines |
| Specificity | WST-1 reduction is proportional to metabolic activity of viable cells; minimal non-enzymatic reduction in fresh medium; dead or metabolically inactive cells produce negligible signal |
| Reaction Conditions / Protocol | Add 10 μL WST-1 reagent per 100 μL culture medium; incubate at 37°C with 5% CO2 for 30 minutes to 4 hours; shake plate gently for 1 minute; measure absorbance at 440 nm with reference at 650 nm; compare treated wells with untreated controls |
| Components / Formulation | Ready-to-use WST-1 solution (WST-1 and mPMS coupling reagent in PBS), provided as sterile-filtered amber solution |
| Storage Conditions | 2–8°C protected from light; stable for 12 months; warm to room temperature and protect from light during assay; avoid prolonged exposure to bright light |
| Shelf Life | 12 months from date of manufacture |
| Package Specifications | 2 mL (200 tests in 96-well format), 25 mL (2,500 tests) |
| Product Form | Sterile ready-to-use liquid reagent |
| Quality Control | Each lot tested against reference cell lines (CHO, HeLa) to confirm proportional signal with increasing cell numbers; sterility verified; reagent blank absorbance <0.1 OD at 440 nm |
| Key Features | Single-reagent addition without preparation or mixing steps; water-soluble formazan allows direct measurement in culture medium; more sensitive than XTT; can be used for time-course kinetic measurements |
For research use only, not for clinical use.
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