CFDA SE Cell Proliferation and Cell Tracking Reagent
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CFDA SE Cell Proliferation and Cell Tracking Reagent

Cat.No: CATR-HMM-0021 Datasheet

Specification Quantities

500T:
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1000T:
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Product Details Related Products
Product Name CFDA SE Cell Proliferation and Cell Tracking Reagent
Catalog No. CATR-HMM-0021
Description This product is a reagent for cell tracing and proliferation detection based on CFDA, SE, consisting of CFDA, SE powder, solvent, and related cell staining buffer. The primary working principle of this reagent is as follows: CFDA, SE has cell membrane permeability and does not inherently emit fluorescence. Upon entering live cells via passive transport through the cell membrane, it is catalyzed by cytoplasmic esterases to form carboxyfluorescein succinimidyl ester (CFSE), which emits strong green fluorescence, cannot penetrate the cell membrane, and remains intact within the cell. CFSE can also spontaneously and irreversibly bind to amino groups within the cell, thereby coupling to cellular proteins. Excess CFDA, SE that has not been coupled is passively diffused back into the extracellular culture medium and removed during subsequent washing steps. Fluorescence in non-dividing cells labeled with CFDA, SE is highly stable, with stable labeling lasting for months, making it ideal for cell colony analysis.
Application This product is commonly used for lymphocyte proliferation assays and can also be used for proliferation assays of other cell types such as fibroblasts, natural killer cells, and hematopoietic progenitor cells.
Spectral Parameters Ex/Em = 494 nm/521 nm
Applicable Instruments Fluorescence microscope, flow cytometer
Storage Store at -20°C away from light.
Shelf Life 2 years
Features This product is the CFDA, SE Cell Proliferation and Tracing Detection Kit, which includes the solvents required to prepare the CFDA, SE storage solution and the staining buffer for cell labeling, simplifying the pre-experimental preparation work. Additionally, CFDA, SE-labeled cells can typically be completed in 15 minutes; however, the optimal labeling time may vary depending on the cell type and should be determined through experimentation.

For research use only, not for clinical use.

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