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| Product Name | mRNA Interferase MazF (20 U/μL) |
| Catalog No. | IVDRM-0168 |
| Description | MazF is the toxin component of the E. coli toxin-antitoxin system and functions as an ACA-specific endoribonuclease. It cleaves single-stranded RNA at the 5' side of ACA sequences, while leaving double-stranded RNA, double-stranded DNA, and single-stranded DNA intact. This strict sequence and substrate specificity makes MazF a precise tool for targeted mRNA cleavage and RNA analysis. |
| Intended Use | For sequence-specific cleavage of single-stranded RNA at ACA sites, including mRNA cleavage and mRNA interference applications. |
| Principle / Technology | MazF is an endoribonuclease that recognizes the ACA sequence in single-stranded RNA and cleaves the RNA chain at the 5' end of this recognition site. |
| Performance Range / Specifications | Cleavage site: 5'...↓ACA...3'. One unit (U) is defined as the amount of enzyme that completely degrades 1 pmol of the standard substrate (ROX-5'-GATAUACATATCT-eclipse; the underlined region is the RNA sequence) within 10 min at 37°C and pH 7.5. |
| Specificity | ACA-specific cleavage of single-stranded RNA; does not cleave double-stranded RNA, double-stranded DNA, or single-stranded DNA. |
| Reaction Conditions / Protocol | Activity is measured at 37°C and pH 7.5. |
| Storage Conditions | Store at -25°C to -15°C. |
| Shelf Life | 2 years. |
| Package Specifications | 1000 U |
| Product Form | Liquid (solution). |
| Concentration | 20 U/μL |
| Activity / Unit Definition | One unit (U) is defined as the amount of enzyme that completely degrades 1 pmol of the standard substrate (ROX-5'-GATAUACATATCT-eclipse; the underlined region is the RNA sequence) within 10 min at 37°C and pH 7.5. |
| Source / Origin | Toxin protein of the E. coli toxin-antitoxin system. |
| Recognition Site | 5'...↓ACA...3' |
For research use only, not for clinical use.
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