Malate Dehydrogenase MDH Enzyme for Metabolite Assays >=15 U/mg 38 kDa Isoelectric Point 5.5 CAS 9001-64-3

Name: Malate Dehydrogenase MDH Enzyme for Metabolite Assays >=15 U/mg 38 kDa Isoelectric Point 5.5 CAS 9001-64-3
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Cat.No: IEC-HMM-0280 Datasheet

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Product Name	Malate Dehydrogenase MDH Enzyme for Metabolite Assays >=15 U/mg 38 kDa Isoelectric Point 5.5 CAS 9001-64-3
Catalog No.	IEC-HMM-0280
Description	Malate dehydrogenase from Alta DiagnoTech reversibly catalyzes the oxidation of L-malate to oxaloacetate with NAD. The enzyme is applied in metabolic indicator assays and as a coupled enzyme in clinical diagnostic kits.
Intended Use	Raw enzyme for metabolite assay kits, kinetic enzyme assays, and research.
Principle / Technology	Oxidizes L-malate to oxaloacetate, reducing NAD to NADH; the reverse reaction reduces oxaloacetate using NADH.
Detection Method	NADH absorbance change at 340 nm.
Sample Type	Serum, plasma, tissue homogenates.
Performance Range / Specifications	Specific activity >=15.0 U/mg. Molecular weight approximately 38 kDa. Isoelectric point 5.5.
Components / Formulation	Malate dehydrogenase lyophilized powder.
Storage Conditions	-20°C.
Shelf Life	Stable for at least 12 months at -20°C.
Package Specifications	1 g per bottle; KU units available.
Product Form	White amorphous powder.
Activity / Unit Definition	>=15.0 U/mg
Molecular Weight	Approximately 38 kDa
Source / Origin	Microbial fermentation.
pH Range / Optimal pH	Optimal pH around 6.0; stable between pH 5.0-9.0.
Shipping Conditions	Cold chain shipment.
Expiration Date / Stability	-20°C stable for one year.
Appearance	White amorphous powder.
CAS Number	9001-64-3
Isoelectric Point	5.5
Inhibitors	Not inhibited by sodium azide.
Optimal Temperature	40°C.
Thermal Stability	Stable below 50°C.
Handling Precaution	Reconstitute in recommended buffer and avoid repeated freezing and thawing.
Michaelis Constant	4.7×10^-5 M (L-Malate); 6.4×10^-5 M (Oxaloacetate); 2.5×10^-5 M (NADH)

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For research use only, not for clinical use.