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| Product Name | M-MLV RNase H- Reverse Transcriptase |
| Catalog No. | IEC-HMM-0090 |
| Description | An RNase H-deficient M-MLV reverse transcriptase engineered to retain polymerase activity while supporting efficient synthesis of longer first-strand cDNA. |
| Intended Use | First-strand cDNA synthesis; full-length cDNA library preparation; 5′ RACE; reverse transcription of longer RNA templates. |
| Principle / Technology | A point-mutated M-MLV enzyme with reduced RNase H activity synthesizes complementary DNA from RNA and retains terminal transferase activity for template-switching workflows. |
| Sample Type | RNA templates. |
| Performance Range / Specifications | Supports cDNA synthesis up to approximately 5 kb and reliable reverse transcription from RNA inputs above 100 ng. |
| Reaction Conditions / Protocol | Use in a reverse-transcription reaction with an appropriate primer, dNTPs and reaction buffer according to the intended downstream workflow. |
| Components / Formulation | RNase H-deficient M-MLV reverse transcriptase preparation. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 10,000 U. |
| Product Form | Reverse transcriptase enzyme solution. |
| Key Features | Reduced RNase H activity; preserved polymerase activity; terminal transferase activity; suitable for longer cDNA synthesis and template-switching applications. |
| Compatibility | Compatible with first-strand cDNA synthesis and 5′ RACE workflows. |
For research use only, not for clinical use.
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