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| Product Name | Lyticase (10 U/μL) |
| Catalog No. | IVDRM-0181 |
| Description | Lyticase is a fungal cell-lysing enzyme widely used in downstream experiments that require hydrolysis of yeast cell walls, including genomics research, yeast artificial chromosome (YAC) extraction, yeast transformation, protoplast preparation, yeast cell disruption, industrial production, and yeast inactivation. This product is supplied as a solution at 10 U/μL, with a commonly used working concentration of 0.15 U/μL. The optimal working concentration should be optimized according to the specific application, yeast species, and cell amount. For yeast genomic DNA or plasmid DNA extraction, the enzyme can be used at the concentration specified in the corresponding kit. |
| Intended Use | Hydrolysis of yeast cell walls for genomics research, yeast artificial chromosome (YAC) extraction, yeast transformation, protoplast preparation, yeast cell disruption, industrial production, and yeast inactivation. |
| Principle / Technology | A fungal cell-lysing enzyme (β-1,3-glucanase-type lytic activity) that hydrolyzes yeast cell walls to lyse cells or generate protoplasts. |
| Sample Type | Yeast and other fungal cells. |
| Performance Range / Specifications | Supplied at 10 U/μL. Commonly used working concentration: 0.15 U/μL. Broad-spectrum lytic enzyme for fungal cells. Stable activity, convenient for storage and transport, with consistent product quality. High efficiency for nucleic acid purification and for obtaining high-quality protoplasts. |
| Specificity | Broad-spectrum lytic activity against fungal cell walls, widely used for yeast cell lysis and protoplast preparation. |
| Reaction Conditions / Protocol | The commonly used working concentration is 0.15 U/μL. Optimize the working concentration according to the specific application, yeast species, and cell amount. For yeast genomic DNA or plasmid DNA extraction, use the concentration specified in the corresponding kit. |
| Components / Formulation | Lyticase solution at 10 U/μL. |
| Storage Conditions | Store at -20°C. |
| Package Specifications | 1500 U; 3000 U; 10000 U |
| Product Form | Solution |
| Concentration | 10 U/μL |
| Source / Origin | Arthrobacter luteus |
| Shipping Conditions | Shipped with ice packs. |
| Application Notes / Precautions | Derived from Arthrobacter luteus. Optimize the working concentration for each application, yeast species, and cell amount; 0.15 U/μL is a commonly used starting point. |
| Batch-to-Batch Consistency | Stable enzymatic activity and consistent product quality, convenient for storage and transport. |
For research use only, not for clinical use.
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