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| Product Name | Lysozyme with Optimized Buffer |
| Catalog No. | IEC-HMM-0119 |
| Description | A lysozyme preparation supplied with an optimized buffer for bacterial cell-wall digestion. The enzyme hydrolyzes the beta-1,4 glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine, promoting lysis of susceptible bacteria. |
| Intended Use | Pretreatment and digestion of Gram-positive bacterial cell walls during DNA and RNA isolation. |
| Principle / Technology | Enzymatic hydrolysis of peptidoglycan beta-1,4 glycosidic bonds, converting insoluble cell-wall polysaccharides into soluble fragments. |
| Detection Method | Enzymatic bacterial cell-wall lysis. |
| Sample Type | Gram-positive bacterial cells. |
| Performance Range / Specifications | 200 mg lysozyme with 10 mL optimized buffer. |
| Specificity | Most suitable for digestion of Gram-positive bacterial cell walls. |
| Reaction Conditions / Protocol | Suspend bacterial cells in the optimized buffer, add lysozyme and incubate according to the downstream nucleic-acid isolation workflow. |
| Components / Formulation | Lysozyme powder, 200 mg; optimized lysozyme buffer, 10 mL. |
| Storage Conditions | Store lysozyme powder at 2-8°C and the buffer at 15-25°C. |
| Package Specifications | 200 mg enzyme with 10 mL buffer. |
| Product Form | Lyophilized enzyme powder with liquid buffer. |
| Key Features | Optimized buffer included; simple and efficient bacterial pretreatment; suitable for Gram-positive cell-wall digestion. |
| Compatibility | Bacterial genomic DNA and total RNA isolation workflows. |
| Application Notes / Precautions | Use the recommended cell density and incubation conditions to avoid incomplete lysis or excessive sample viscosity. |
For research use only, not for clinical use.
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