| Product Name | EdU Imaging Reagents (Red Fluorescence) |
| Catalog No. | CATR-HMM-0020 |
| Description | Cell proliferation detection is a basic experimental method for evaluating cell health, genetic toxicity, and the effects of anticancer drugs. The commonly used method for detecting cell proliferation is the BrdU method. The EdU method represents an upgrade and breakthrough over the BrdU method. EdU (5-bromo-2'-deoxyuridine) is a pyrimidine analog that can be incorporated into the DNA double helix during DNA synthesis. The EdU method is based on the “click” reaction, an atom-to-atom covalent reaction between azide compounds and alkyne catalysed by copper. |
| Application | This product can detect cell proliferation and cell cycle analysis, suitable for fluorescence microscopy and flow cytometry detection. |
| Applicable Instruments | Fluorescence microscope, flow cytometer |
| Storage | Store at -20°C away from light. |
| Shelf Life | 1 year |
| Features | The EdU reagent in the kit contains acetylene, while the Yefluor 594 Azide dye reagent contains azide compounds. The click method for EdU labeling is rapid, effective, and easy to use. Only a small amount of azide dye is required to effectively label integrated EdU. Standardized glutaraldehyde fixation and detergent permeabilization enable detection reagents to enter cells, whereas the Brdu method requires DNA denaturation (such as acid denaturation, heat denaturation, or DNase digestion) to expose Brdu for antibody binding. |
| Notes | Ensure that the reagents are colorless when used; do not use additives or buffers that have turned yellow. To ensure that the Click reaction reagents can reach the nucleus, the cells need to be adequately fixed and permeabilized. Since copper ions can bind to certain reagents, this may reduce the effective concentration required to catalyze the Click reaction. Therefore, do not introduce metal chelating agents (e.g., EDTA, EGTA, citrate salts, etc.) into any buffers or reagents prior to the Click reaction. For certain experimental samples containing these substances, additional washing steps may be required before performing the Click reaction; The Click reaction is only effective when copper ions are in the appropriate oxidation state. The copper ions used in the Click reaction are divalent copper (Cu²⁺). Extending the Click reaction time beyond 30 minutes does not improve the signal. It is recommended to use fresh Click reaction reagents for another 30-minute incubation to more effectively enhance labeling efficiency. |
For research use only, not for clinical use.
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