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| Product Name | LDH Cytotoxicity Detection Kit |
| Catalog No. | CATR-HMM-0037 |
| Description | A lactate dehydrogenase (LDH) release-based colorimetric kit for quantifying cell membrane integrity and cytotoxicity. Damaged or dying cells release LDH into the culture supernatant, which is detected through an enzymatic coupling reaction that converts tetrazolium salt to a red formazan product. |
| Intended Use | Quantitative assessment of cytotoxicity, cell death, and membrane damage induced by chemical compounds, biological toxins, immune effector cells, or environmental stress in drug safety screening and immunology research. |
| Principle / Technology | Released LDH catalyzes the interconversion of lactate and pyruvate with concomitant reduction of NAD+ to NADH; NADH then reduces a tetrazolium salt (INT) to a red formazan via diaphorase, measured at 490 nm in a coupled reaction. |
| Detection Method | Colorimetric absorbance measurement at 490 nm, with reference wavelength at 600–690 nm |
| Sample Type | Conditioned culture supernatant from adherent or suspension cells grown in serum-containing or serum-reduced medium; also applicable to serum, plasma, and tissue homogenates |
| Performance Range / Specifications | Detection range: 0.1–10% cytotoxicity relative to maximum LDH release control; linear between 0.19–3.125 mU/mL LDH activity |
| Sensitivity / LOD | Equivalent to approximately 200–500 dying cells per well for typical adherent lines |
| Specificity | LDH enzyme activity is specific to damaged or lysed cells; intact viable cells retain LDH intracellularly; assay conditions optimized to minimize spontaneous LDH release from healthy cells |
| Reaction Conditions / Protocol | Transfer 100 μL cell culture supernatant to a new 96-well plate; add 100 μL reaction mixture (catalyst and dye solution freshly mixed); incubate 30 minutes at room temperature in the dark; stop reaction with 50 μL 1 M HCl if needed; measure absorbance at 490 nm |
| Components / Formulation | Catalyst Solution (diaphorase/NAD+ mixture, lyophilized), Dye Solution (INT and sodium lactate), Lysis Solution (1% Triton X-100 for maximum release controls), Reaction Buffer, BSA for low-serum media supplementary control |
| Storage Conditions | -20°C protected from light; reconstituted reagents stable at 2–8°C for 4 weeks |
| Shelf Life | 12 months from date of manufacture |
| Package Specifications | 500 tests, 2,000 tests (96-well microplate format) |
| Product Form | Lyophilized catalyst with separate liquid dye and lysis buffer solutions |
| Quality Control | Each lot tested against reference LDH enzyme standard; background LDH levels verified in low-serum conditions; maximum release control included |
| Key Features | Non-radioactive assay safe for routine laboratory use; does not require cell washing or pre-labeling; compatible with most standard serum-containing media; results available within 30 minutes |
For research use only, not for clinical use.
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